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First published online August 23, 2004
Journal of Experimental Biology 207, 3411-3417 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01134
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Endothelin B receptor Ca2+ signaling in shark vascular smooth muscle: participation of inositol trisphosphate and ryanodine receptors

Susan K. Fellner1,2,* and Laurel A. Parker2

1 Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
2 Mount Desert Island Biological laboratory, Salisbury Cove, ME 04672, USA



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  		Fig. 1. Intracellular Ca2+ ([Ca2+]i) responses of shark VSM to ETBR receptor agonists and endothelin-1 (ET-1). (A) The ETBR agonists IRL 1620 and sarafotoxin S6c (SRX) produce the same increase in [Ca2+]i as ET-1. (B) Representative tracing of the cytosolic calcium responses of shark anterior mesenteric artery VSM. The ETBR agonist IRL 1620 causes a prompt increase in [Ca2+]i. In the continued presence of IRL 1620, addition of ET-1 does not cause any further significant peak increase in [Ca2+]i.

 


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  		Fig. 2. Effect of the IP3R blockers, TMB-8 and 2-APB, on the response of shark VSM to the ETBR agonist IRL 1620 in Ca2+-free Ringer. (A) Representative tracings of the response of VSM to ETBR agonist stimulation in the presence or absence of TMB-8 or 2-APB. (B) Both inhibitors block the [Ca2+]i mobilization response by about 70% (*P<0.01).

 


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  		Fig. 3. Participation of the RyR in the [Ca2+]i response to the ETBR agonist IRL 1620 in shark mesenteric VSM. (A) Representative tracing of the diminished [Ca2+]i response to IRL 1620 in the continued presence of the cADPR antagonist 8-Br cADPR. (B) Typical tracings of the stimulatory effect of a low concentration of ryanodine on the RyR and blocking effect of high concentrations of ryanodine. IRL 1620 was added in the continued presence of ryanodine. (C) Summary data of the response of VSM to a concentration of ryanodine that activates the RyR (5 µmol l-1), followed by response to the ETBR agonist IRL 1620 in the continued presence of ryanodine. The ryanodine and IRL 1620 plus ryanodine results were different from each other (*P<0.05) and both were different from IRL 1620 alone (P<0.05). (D) Three inhibitors of the RyR, 8Br-cADPR, high concentration of ryanodine (100 µmol l-1) and Ruthenium Red, block the response of VSM to the ETBR agonist IRL 1620 by approximately 39% (*P<0.01).

 

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© The Company of Biologists Ltd 2004