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First published online August 6, 2004
Journal of Experimental Biology 207, 3125-3129 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01133
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Role of trehalose phosphate synthase and trehalose during hypoxia: from flies to mammals

Qiaofang Chen and Gabriel G. Haddad*

Department of Pediatrics and Neuroscience, Albert Einstein College of Medicine, Bronx, New York, USA



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  		Fig. 1. Overexpression of tps1 in adult flies increases trehalose level and increases tolerance to anoxia. (A) Flies overexpressing tps1 (E1 and E2) have a shorter recovery time than controls (C1, C2 and C3) after exposure to 5 min anoxia. *P<0.005 as compared with controls. (B) Flies overexpressing tps1 contain more trehalose than controls. *P<0.005 as compared with controls.

 


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  		Fig. 2. Western blot of total (T), soluble (S), and aggregated (A) proteins prepared with the addition of 0, 0.3, 0.5 mol l–1 trehalose in extraction buffer. Flies were subjected to 4 h of anoxia and 5 µg of aggregated proteins were prepared by adding 0, 0.3, 0.5 mol l–1 trehalose. Although the same amount of Na+/K+ATPase seemed to be present in total proteins, much less Na+/K+ATPase was present in the aggregated proteins with 0.5 mol l–1 trehalose as compared with that lacking trehalose. Note that the beta-tubulin concentration did not change in each of T, S or A proteins as a function of trehalose.

 


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  		Fig. 3. Intracellular trehalose reduces ubiquitinated proteins during hypoxia. (A) Western signals of ubiquitinated proteins and ß-actin. (B) Relative amount of ubiquitinated proteins per same amount of total proteins after hypoxic treatment for 0, 1, 2 and 3 days in HEK-v and HEK-dtps1 cells. This figure shows that there was almost no difference in the ratio of ubiquitinated proteins/ß-actin between HEK-v and HEK-dtps1 cells at days 0 and 1 of hypoxia exposure. However, as exposure time increased, there was a significant difference on days 2 and 3. *P<0.05 compared to HEK-dtps1 cells.

 




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