QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online June 16, 2004
Journal of Experimental Biology 207, 2679-2684 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01088

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rallière, C. Articles by Rescan, P.-Y. Search for Related Content PubMed PubMed Citation Articles by Rallière, C. Articles by Rescan, P.-Y. Social Bookmarking                         What's this?

The genes for the helix-loop-helix proteins Id6a, Id6b, Id1 and Id2 are specifically expressed in the ventral and dorsal domains of the fish developing somites

Cécile Rallière, François Chauvigné and Pierre-Yves Rescan^*

SCRIBE-INRA, Campus de Beaulieu, 35042 Rennes, France

View larger version (65K): [in a new window]   Fig. 1. Comparison of amino acid sequence of trout Id6a, Id6b, Id1 (Y08368), Id2 (Y08369) and zebrafish Id6 (AF007414) and Id3 (AY065841). Dark shading indicates identity; light shading indicates similarity. The helix-loop-helix region is indicated.

View larger version (60K): [in a new window]   Fig. 2. Expression of TId6 in trout embryos. The expression was identical using Id6a or Id6b riboprobe. (A) Stage 12 embryo (approximately 20 somites), dorsal view. The label is observed in the tail bud, the neural keel (white arrow), the rostral presomitic mesoderm (arrowhead) and the somites (arrow). (B) Stage 15 embryo (35 somites), lateral view. The label progresses caudally as somites form and is higher in the ventral and dorsal domains of the somites. (C) Stage 20 embryo (the segmentation is complete), lateral view. Axial structures as well as ventral (arrowheads) and dorsal domains of the somites are labelled. Scale bars: A, 230 µm; B, 400 µm; C, 600 µm.

View larger version (93K): [in a new window]   Fig. 3. Expression of TId6 (A), TId1 (B), TId2 (C), troponin C (D) and desmin (E) in stage 15 trout embryos. (A–E) Transverse sections. (A) TId6 transcripts are concentrated in the ventral and dorsal extremes of the somite (arrows) as well as in the neural tube. (B) TId1 transcripts are observed in the dorsal part of the neural tube and in the ventral and dorsal somitic cells (arrows). (C) TId2 transcripts accumulate in the dorsal part of the neural tube, in the pronephros and in dorsal extremes of the somite (arrow). (D,E) Troponin C (D) and desmin (E) transcripts are visualized in the deep part of the somite. S, somite; N, notochord; NT, neural tube; P, pronephros. Scale bars: A–C, 30 µm; D,E, 20 µm.

View larger version (60K): [in a new window]   Fig. 4. Expression of TId6 (A), TId1 (B) and TId2 (C) in the head region of a stage 16 embryo. (A–C) Dorsal views. (A,B) Transcripts for TId6 and TId1 accumulate in the cerebellum, the optic tectum, the telencephalon, the lens and the retina. (C) Transcripts for TId2 accumulate mainly in the telencephalon and the lens. C, cerebellum; OT, optic tectum; T, telencephalon; L, lens; R, retina. Scale bars: A–C, 250 µm.

View larger version (87K): [in a new window]   Fig. 5. Expression of TId1 (A) and TId2 (B) in trout embryo. (A) Stage 16 embryo (45 somites), lateral view. Transcripts for TId1 are localized in the tail bud (arrow), paraxial mesoderm and transiently in the ventral and dorsal extremes of the neoformed somites (arrowheads). (B) Stage 16 embryo (40 somites), lateral view. Transcripts for TId2 concentrate in the dorsal domain of the somites (black arrowheads). A strong signal is also apparent in the pronephros (white arrowheads). Scale bars: A, 300 µm; B, 250 µm.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

Twitter