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First published online April 8, 2004
Journal of Experimental Biology 207, 1607-1613 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.00935
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Stress gene (hsp70) sequences and quantitative expression in Milnesium tardigradum (Tardigrada) during active and cryptobiotic stages

Ralph O. Schill1,*, Günther H. B. Steinbrück2 and Heinz-R. Köhler1

1 Animal Physiological Ecology, Zoological Institute, University of Tübingen, Konrad-Adenauer-Str. 20, D-72072 Tübingen, Germany
2 Molecular Biology, Institute for Cell Biology, Auf der Morgenstelle 28, D-72076 Tübingen, Germany



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  		Fig. 1. SEM images of the tardigrade Milnesium tardigradum. (A) Active hydrated individual, stage I. (B) Dehydrated, cryptobiotic individual (`tun'), stage III.

 


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  		Fig. 2. hsp70 expression at the different life cycle stages (I-V) of the tardigrade Milnesium tardigradum: (A) hsp70 isoform 1, (B) hsp70 isoform 2 and (C) hsp70 isoform 3. The mRNA copy number was calculated based on the beta-actin housekeeping gene as described in the Materials and methods. Relative expression levels refer to stage I=100%=1.0. Results are presented as means ± S.D. The stages are as follows: I, active before cryptobiosis; II, transitional before cryptobiosis; III, cryptobiotic; IV, transitional after cryptobiosis; V, active after cryptobiosis.

 


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  		Fig. 3. hsp70 expression of the control active stage (I) and an active stage subjected to a heat shock (hs) at 37°C for 90 min: (A) hsp70 isoform 1, (B) hsp70 isoform 2 and (C) hsp70 isoform 3. The mRNA copy number was calculated based on the beta-actin housekeeping gene as described in the Materials and methods. Results are presented as means ± S.D.

 




© The Company of Biologists Ltd 2004