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Regulation of Na+/K+-ATPase activity by nitric oxide in the kidney and gill of the brown trout (Salmo trutta)

Christian K. Tipsmark and Steffen S. Madsen*

Institute of Biology, University of Southern Denmark, Odense University, Campusvej 55, DK-5230 Odense M, Denmark



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  		Fig. 1. The effect of sodium nitroprusside (SNP) concentration on in situ Na+/K+-ATPase activity (measured as ouabain-sensitive Rb+ uptake) in kidney (A) and gill (B) tissue. Samples were pre-incubated for 10 min with the dose indicated before the Rb+ uptake assay. Different letters indicate significant difference (P<0.05). Values are means ± S.E.M. (N=8).

 


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  		Fig. 2. The effect of pre-incubation time with sodium nitroprusside (SNP) on in situ Na+/K+-ATPase activity (measured as ouabain-sensitive Rb+ uptake) in kidney (A) and gill (B) tissue. The samples were pre-incubated with SNP (1 mmol l–1) for the time indicated before the Rb+ uptake assay. Different letters indicate significant difference (P<0.05). Values are means ± S.E.M. (N=8).

 


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  		Fig. 3. The effect of the nitric oxide (NO) donors sodium nitroprusside (SNP; 1 mmol l–1) and papa-nonoate (NOC-15; 0.2 mmol l–1) on in situ Na+/K+-ATPase activity (measured as ouabain-sensitive Rb+ uptake) in kidney (A) and gill (B) tissue. Samples were pre-incubated for 10 min with the NO donors before the Rb+ uptake assay. Different letters indicate significant difference (P<0.05). Values are means ± S.E.M. (N=8).

 


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  		Fig. 4. The effect of sodium nitroprusside (SNP; 0.5 mmol l–1) on in situ Na+/K+-ATPase activity (measured as ouabain-sensitive Rb+ uptake) in kidney (A) and gill (B) tissue incubated in the presence or absence of the nitric oxide scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO; 1 mmol l–1). Samples were pre-incubated for 10 min before the Rb+ uptake assay. Different letters indicate significant difference (P<0.05). Values are means ± S.E.M. (N=8).

 


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  		Fig. 5. Effect of sodium nitroprusside (SNP; 1 mmol l–1) and dibutyryl cyclic GMP (db-cGMP; 1 mmol l–1) on in situ Na+/K+-ATPase activity (measured as ouabain-sensitive Rb+ uptake) in kidney (A) and gill (B) tissue. Samples were pre-incubated for 10 min before the Rb+ uptake assay. Different letters indicate significant difference (P<0.05). Values are means ± S.E.M. (N=8).

 

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