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Myogenic cell cycle duration in Harpagifer species with sub-Antarctic and Antarctic distributions: evidence for cold compensation

Julie C. Brodeur1,*, Jorge Calvo2, Andrew Clarke3 and Ian A. Johnston1

1 Gatty Marine Laboratory, School of Biology, Division of Environmental and Evolutionary Biology, University of St Andrews, St Andrews, Scotland, KY16 8LB, UK
2 Centro Austral de Investigaciones Cientificas (CADIC), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), CC92, Ushuaia, 9410, Tierra del Fuego, Argentina
3 British Antarctic Survey, High Cross, Madingley Road, Cambridge, CB3 OET, UK



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  		Fig. 1. Theoretical curve obtained during repeated S-phase labelling. See text for details.

 


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  		Fig. 2. Section from the fast myotomal muscle of Harpagifer antarcticus illustrating nuclei stained uniquely for c-met (black arrow) and nuclei doubly stained for c-met and 5-bromo-2'-deoxyuridine (BrdU; red arrow).

 


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  		Fig. 3. Proportion of c-met positive muscle cells immunoreactive for 5-bromo-2'-deoxyuridine (BrdU) following repeated intraperitoneal injections of BrdU in Harpagifer antarcticus acclimated to 0°C (black triangles; N=12) and Harpagifer bispinis acclimated to 5°C (blue circles; N=18) and 10°C (red circles; N=15).

 




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