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Lactate efflux from sarcolemmal vesicles isolated from rainbow trout Oncorhynchus mykiss white muscle is via simple diffusion

Rainie L. Sharpe^* and C. Louise Milligan

Department of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7
^* Present address: Department of Biology, University of New Brunswick (Saint John), PO Box 5050, Tucker Park Road, Saint John, New Brunswick, Canada, E2L 4M5

View larger version (9K): [in a new window]   Fig. 1. Lactate loss (pmol mg-1 protein) from rainbow trout white muscle sarcolemmal vesicles over time. Vesicles were loaded with 25 mmol l-1 lactate in 140 mmol l-1 KCl/Mops + 18.5 kBq 14C-lactate. Values are means ± 1 S.E.M.; N=6 (10, 15, 20, 40, 50 and 70 s values); N=10 (30, 60 s values); N=5 (100, 300 s values).

View larger version (11K): [in a new window]   Fig. 2. The effect of internal lactate concentration on lactate efflux from trout white muscle sarcolemmal vesicles. All efflux was measured over a period of 1 min. N=7 (50 and 100 mmol l-1 lactate concentrations); N=6 for 10 mmol l-1 lactate); N=4 (300 mmol l-1 lactate); N=12 (25 mmol l-1 lactate).

View larger version (31K): [in a new window]   Fig. 3. (A) The effects of various inhibitors on lactate efflux from trout white muscle sarcolemmal vesicles. All vesicles were loaded with 25 mmol l-1 lactate and 18.5 kBq 14C-lactate. Values are means ± 1 S.E.M. Control (N=5): efflux was measured in KCl/Mops buffer; STOP (N=10), efflux in ice-cold, 2.5 mmol l-1 HgCl2; 75 mmol l-1 Na (N=7): efflux in 75 mmol l-1 KCl, 75 mmol l-1 NaCl Mops buffer; 5 mmol l-1 CIN (N=6): efflux in KCl/Mops containing 5 mmol l-1 -cyano-4-hydroxycinnamate; 5 mmol l-1 SITS (N=11): efflux in KCl/Mops containing 5 mmol l-1 4-acetoamido-4'-isothyiocyanstilbene-2,2'-disulphonic acid. *A significant difference (P<0.05) from the control value. (B) The influence of pH gradient on lactate efflux from trout white muscle sarcolemmal vesicles. Values are means ± 1 S.E.M. pHi<pHe, N=11; pHi>pHe, N=7, pHi=pHe=5.1, N=5; pHi=pHe=7.4, N=6. *A significant difference (P<0.05) from the pHi=pHe=7.4 value.