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First published online October 27, 2003
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Catecholamine secretion in trout chromaffin cells experiencing nicotinic receptor desensitization is maintained by non-cholinergic neurotransmission

Brian McNeill, Colin J. Montpetit and Steve F. Perry*

Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario K1N 6N5, Canada



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  		Fig. 1. (A) The effects of continuous infusion of control saline (unfilled bars; N=8) or rapidly switching to saline containing nicotine (10–5 mol l–1; filled bars; N=8) or nicotine plus hexamethonium (10–3 mol l–1; cross-hatched bars; N=8) on catecholamine secretion in an in situ perfused posterior cardinal vein preparation of rainbow trout Oncorhynchus mykiss. (B) After 10 min, the preparations were electrically stimulated using a frequency of 20 Hz and maximal catecholamine secretion rates were determined under the various conditions. An asterisk denotes a statistically significant difference from the `pre-switch' (Pre) value; a dagger represents a significant difference from the secretion rate after 10 min; a double dagger represents a significant difference from the control (saline-perfused) group.

 


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  		Fig. 2. Total catecholamine (adrenaline plus noradrenaline) levels in several tissues that could potentially contribute to catecholamine secretion in the perfused posterior cardinal vein (PCV) preparation; N=6.

 


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  		Fig. 3. (A) The effects of continuous infusion of control saline (unfilled bars; N=8) or rapidly switching to saline containing nicotine (10–5 mol l–1; filled bars; N=8) or nicotine plus a cocktail of 10–3 mol l–1 hexamethonium and 10–5 mol l–1 atropine (cross-hatched bars; N=8) on catecholamine secretion in an in situ perfused posterior cardinal vein preparation of rainbow trout Oncorhynchus mykiss. (B) After 10 min, the preparations were electrically stimulated using a frequency of 1 Hz and maximal catecholamine secretion rates were determined under the various conditions. An asterisk denotes a statistically significant difference from the `pre-switch' (Pre) value; a dagger represents a significant difference from the secretion rate after 10 min.

 


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  		Fig. 4. (Ai,Bi) The effects of rapidly switching from control saline to saline containing nicotine (10–5 mol l–1; hatched bars; N=18) or nicotine plus the VPAC receptor antagonist VIP6-28 (10–6 mol l–1; filled bars; N=18) on catecholamine secretion in an in situ perfused posterior cardinal vein preparation of rainbow trout Oncorhynchus mykiss. After 10 min, the preparations were electrically stimulated using a frequency of either (Aii) 20 Hz or (Bii) 1 Hz and maximal catecholamine secretion rates were determined under the various conditions. An asterisk denotes a statistically significant difference from the `pre-switch' (Pre) value; a dagger represents a significant difference from the secretion rate after 10 min; a double dagger represents a significant difference from the control (salineperfused) group.

 


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  		Fig. 5. The effects of (Aii) cVIP (10–6 mol l–1; N=15), (Bii) Ang II (10–5 mol l–1; N=6) and (Cii) methylcholine (10–3 mol l–1; N=6) on catecholamine secretion rates (Ai–Ci) in posterior cardinal vein preparations previously perfused with control saline (hatched bars) or after rapidly switching to saline containing nicotine (10–5 mol l–1; filled bars). An asterisk denotes a statistically significant difference from the `preswitch' (Pre) value; a dagger represents a significant difference from the secretion rate after 10 min; a double dagger represents a significant difference from the control (saline-perfused) group. Agonist inj. indicates rate measured after agonist injection (see Materials and methods).

 

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© The Company of Biologists Ltd 2003