spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online October 10, 2003
This Article
Right arrow Summary Freely available
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Watson, R. R.
Right arrow Articles by Davis, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Watson, R. R.
Right arrow Articles by Davis, R. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Immunohistochemical fiber typing of harbor seal skeletal muscle

Rebecca R. Watson1,*, Todd A. Miller2 and Randall W. Davis1

1 Texas A&M University, Galveston, TX 77551, USA
2 Department of Kinesiology, Texas A&M University, College Station, TX 77840, USA



View larger version (9K):

[in a new window]
  		Fig. 1. Schematic diagram illustrating the location of the three transverse sections taken from the epaxial muscles. CR, cranial; MID, middle; CA, caudal.

 


View larger version (16K):

[in a new window]
  		Fig. 2. Representative transverse section showing the coring pattern of samples collected from the epaxial muscles. CA, caudal.

 


View larger version (103K):

[in a new window]
  		Fig. 5. Representative serial cross sections from the epaxial muscles of a harbor seal Phoca vitulina stained for myosin heavy chain isoforms using a series of monoclonal antibodies. (A) Type I (slow-twitch, oxidative) fibers, (B) type IIa (fast-twitch, oxidative) fibers and (C) type IIb (fast-twitch, glycolytic) fibers. Lack of stain in C indicates an absence of type IIb fibers in this cross section of the muscle. Bar, 50 µm.

 


View larger version (36K):

[in a new window]
  		Fig. 3. Silver stain (reverse western blot) of (A) rat vastus medialis muscle, (B) seal epaxial muscles, and (C) seal pectoralis muscle, showing differentiation of myosin heavy chain isoforms based on molecular mass. The type IIb band is absent in both seal muscles.

 


View larger version (16K):

[in a new window]
  		Fig. 4. Representative transverse section showing the statistical division used for testing homogeneity within transverse sections of the epaxial muscles. The broken line represents the natural vertical axis of the animal and the solid line the line drawn at a 45° angle to the vertical axis to divide the epaxial muscles into proximal (P) and distal (D) sections. CA, caudal.

 

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



© The Company of Biologists Ltd 2003