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First published online September 23, 2003

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Trypsin-modulating oostatic factor: a potential new larvicide for mosquito control

D. Borovsky

University of Florida-IFAS, Florida Medical Entomology Laboratory, 200 9th Street, SE Vero Beach, FL 332962,

View larger version (49K): [in a new window]   Fig. 1. A three-dimensional NMR model of Aedes aegypti trypsin-modulating oostatic factor (Aea-TMOF). A left-handed helix of six prolines can be observed at the C-terminus. The N-terminus with the first four amino acid sequence (YDPA) that is important in binding to the TMOF gut receptor is underlined.

View larger version (121K): [in a new window]   Fig. 2. Immunolocalization of trypsin-modulating oostatic factor (TMOF) binding to mosquito midgut receptor 48 h after the blood meal. (A) A transverse section (7 µm) was incubated with TMOF and polyclonal antibodies. Distinct binding of TMOF to its receptor was observed on a small area of the transverse section (dark area between arrows). (B). Fourfold magnification of the binding region. PM, peritrophic membrane; Lu, gut lumen; He, hemolymph side of the gut.

View larger version (50K): [in a new window]   Fig. 3. Effect of feeding Aedes aegypti trypsin-modulating oostatic factor (Aea-TMOF) on larval growth and development. Mosquito larvae were fed Brewer's yeast (20 µg) with either (A) 188 µg TMOF or (B) no TMOF. Larval growth and development were compared after 6 days.