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Regulation of branchial Na+/K+-ATPase in common carp Cyprinus carpio L. acclimated to different temperatures

Juriaan R. Metz, Erwin H. van den Burg, Sjoerd E. Wendelaar Bonga and Gert Flik*

Department of Animal Physiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands



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  		Fig. 1. Specific branchial Na+/K+-ATPase activity (Vspec) in carp acclimated for 8 weeks to water temperatures of 15, 22 or 29°C, and in 22°C-acclimated carp that had a minipump filled with vehicle or cortisol implanted for 7 days. Activity was assayed at optimal temperature (Vmax; white bars) and at the acclimation temperature of the fish (Vapparent; grey bars). Values are means ± S.E.M. Asterisks indicate statistically significant differences among groups (*P<0.05; **P<0.01).

 


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  		Fig. 2. Arrhenius plot of the specific branchial Na+/K+-ATPase activity (Vspec) of carp acclimated to water temperatures of 15, 22 or 29°C. Q10-values are indicated in the graph. Breaks in the plot occur at 29°C in the 15°C-acclimated fish and at 22°C in the plot of the 22°C-acclimated fish. No apparent break in the plot of the 29°C fish is observed over the range of the temperatures tested. Values are means ± S.E.M.

 


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  		Fig. 3. Immunohistochemical demonstration of Na+/K+-ATPase immunoreactive cells (chloride cells) in gills of (A) 15°C-acclimated and (B) 29°C-acclimated carp. Chloride cells are more abundant and larger in the gills of 15°C-acclimated carp. Representative examples are shown at the same magnification. Scale bars, 100 µm.

 


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  		Fig. 4. (A) Plasma cortisol levels in carp acclimated for 8 weeks to water temperatures of 15, 22 or 29°C and in 22°C-acclimated carp that had a minipump filled with vehicle or cortisol implanted for 7 days. (B) Pituitary prolactin expression, relative to the housekeeping gene ß-actin, in 15, 22 or 29°C-acclimated carp quantified by real-time PCR. Values are means ± S.E.M. Asterisks indicate statistically significant differences among groups (*P<0.05; **P<0.01).

 

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© The Company of Biologists Ltd 2003