QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sötz, E. Articles by Pelster, B. Search for Related Content PubMed PubMed Citation Articles by Sötz, E. Articles by Pelster, B.

Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla

E. Sötz, H. Niederstätter and B. Pelster^*

Institut für Zoologie und Limnologie, Universität Innsbruck, A-6020 Innsbruck, Austria

View larger version (12K): [in a new window]   Fig. 1. Changes in intracellular pH (pHi) at varied extracellular pH (pHe) in swimbladder gas gland cells. The values in parentheses are the number of cell cultures measured. Values are means ± S.E.M.

View larger version (26K): [in a new window]   Fig. 2. The influence of 5-(N-methyl-N-isobutyl)-amiloride (MIA; 10-5 mol l-1) on intracellular pH (pHi) of gas gland cells at a constant extracellular pH (pHe) of 7.4 (A) and following an artificial intracellular acidification induced using the ammonium pulse technique (10 mmol l-1 NH4Cl in phosphate-buffered saline for 8 min) (B). *Highly significantly different from the control (P<0.01). Values are means ± S.E.M.

View larger version (23K): [in a new window]   Fig. 3. The influence of 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS; 10-4 mol l-1) on intracellular pH (pHi) of gas gland cells at a constant extracellular pH (pHe) of 7.4 (A) and following an artificial intracellular acidification induced using the ammonium pulse technique (10 mmol l-1 NH4Cl in phosphate-buffered saline for 8 min) (B). Significantly different from the control (P<0.05); *highly significantly different from the control (P<0.01). Values are means ± S.E.M.

View larger version (23K): [in a new window]   Fig. 4. The influence of bafilomycin A1 (10-5 moll-1) on intracellular pH (pHi) of gas gland cells following an artificial intracellular acidification induced using the ammonium pulse technique (10 mmoll-1 NH4Cl in phosphate-buffered saline for 8 min, pHe=7.4). Significantly different from the control (P<0.05); *highly significantly different from the control (P<0.01). Values are means ± S.E.M.

View larger version (24K): [in a new window]   Fig. 5. Hypothetical model showing the various proton-translocating mechanisms identified so far in swimbladder gas gland cells of the European eel. Acidic metabolites (lactic acid and CO2) are produced in the glycolytic pathway and in the pentose phosphate shunt. This acid is secreted at the basolateral membranes to reduce the effective gas-transport capacity of the blood via the Root effect and the salting out effect (see Pelster, 1997; Pelster and Randall, 1998). The decrease in gas-transport capacity induces an increase in gas partial pressures in the blood, which provides the necessary partial pressure gradient towards the swimbladder lumen so that gases can enter the swimbladder by diffusion. The mechanisms of lactate transport have not yet been characterized in detail. CA, membrane-bound carbonic anhydrase.