Electrospray ionisation mass spectrometric analysis of lipid restructuring in the carp (Cyprinus carpio L.) during cold acclimation
S. Brooks1,
G. T. Clark2,
S. M. Wright2,
R. J. Trueman3,
A. D. Postle2,
A. R. Cossins3,* and
N. M. Maclean1
1 Department of Cell Sciences, School of Biological Sciences, University of
Southampton, Southampton, SO16 7PX, UK
2 Child Health, Division of Infection, Inflammation and Repair, School of
Medicine, Southampton General Hospital, Southampton, SO16 6YD, UK
3 Integrative Biology Research Division, School of Biological Sciences,
University of Liverpool, Liverpool, L69 7ZB, UK
View larger version (26K):
[in a new window]
|
Fig. 1. Electrospray ionisation mass spectra showing molecular species composition
of carp liver microsome phospholipids. As phosphatidylcholine (PC) species
were partially sodiated under positive ionisation conditions (spectra not
shown), they were analysed from precursor scans of m/z 184 (A) that
selectively detected [M+H]+ molecular ions. Acidic phospholipids
were detected as molecular ions under negative ionisation conditions (B). As
plasmenyl phosphatidylethanolamine (PE) species were not detected in this
spectrum, PE species were quantified as sodium adducts from constant neutral
loss scans of m/z 141 under positive ionisation conditions (C) after
the addition of 5 mmol l-1 NaI. Such neutral loss scans selectively
detected diacyl but not alkenylacyl PE species. Phosphatidylinositol (PI)
species were identified from precursor scans of m/z 241 (D), while
phosphatidylserine (PS) and phosphatidylglycerol (PG) were identified from
constant neutral loss scans of m/z 87 and precursor scans of
m/z 153, respectively (data not shown).
|
|
View larger version (16K):
[in a new window]
|
Fig. 2. Changes in fatty acid saturation of the molecular species components of the
three phospholipid classes, (A) phosphatidylcholine (PC), (B)
phosphatidylethanolamine (PE) and (C) phosphatidylinositol (PI), isolated from
carp liver microsomes. Fish were kept at each temperature for five days before
being killed. Values represent means ± S.D. (N=3-4
individuals). A significant difference from the control value (30°C) is
indicated by an asterisk [P 0.05; one-way analysis of variance
(ANOVA)].
|
|
View larger version (22K):
[in a new window]
|
Fig. 3. The effect of temperature acclimation on the individual molecular species
composition of the phosphatidylcholine fraction from carp liver microsomes.
Results are expressed as mol % total phosphatidylcholine (means ± S.D.,
N=3-4 individuals). A significant difference from the control value
(30°C) is indicated by an asterisk [P0.05; one-way analysis
of variance (ANOVA)].
|
|
View larger version (23K):
[in a new window]
|
Fig. 4. The effect of temperature acclimation on the individual molecular species
composition of the phosphatidylethanolamine fraction from carp liver
microsomes. Results are expressed as mol % total phosphatidylethanolamine
(means ± S.D., N=3-4 individuals). A significant difference
from the control value (30°C) is indicated by an asterisk
[P0.05; one-way analysis of variance (ANOVA)].
|
|
View larger version (23K):
[in a new window]
|
Fig. 5. The effect of temperature acclimation on the individual molecular species
composition of the phosphatidylinositol fraction from carp liver microsomes.
Results are expressed as mol % total phosphatidylinositol (means ±
S.D., N=3-4 individuals). A significant difference from the control
value (30°C) is indicated by an asterisk [P0.05; one-way
analysis of variance (ANOVA)].
|
|
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
© The Company of Biologists Ltd 2002
