QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Behrens, J. W. Articles by Weber, R. E. Search for Related Content PubMed PubMed Citation Articles by Behrens, J. W. Articles by Weber, R. E.

The archaeogastropod mollusc Haliotis iris: tissue and blood metabolites and allosteric regulation of haemocyanin function

Jane W. Behrens^1,3, John P. Elias^2,3, H. Harry Taylor³ and Roy E. Weber^1,*

¹ Department of Zoophysiology, Institute Biological Sciences, University of Aarhus, DK 8000 Aarhus, Denmark,
² School of Biological Sciences, Monash University, Clayton, Victoria 3800, Australia and
³ Department of Zoology, University of Canterbury, Private Bag 4800, Christchurch, New Zealand

View larger version (15K): [in a new window]   Fig. 1. The concentrations of tauropine (A) and D-lactate (B) in the haemolymph (AO, aorta; PS, pedal sinus; AH, adductor haemocoel; mmol l–1) and the tissues (FM, foot muscle; AM, adductor muscle; mmol kg–1 fresh mass) in Haliotis iris settled in water (open columns) or emersed in air for 24 h (filled columns) at 15°C. Values are means + S.E.M.; see Table 1 for N values.

View larger version (18K): [in a new window]   Fig. 2. The effects of dialysis against various ions on the O2-affinity (logP50) of Haliotis iris haemocyanin at 15°C, pH 7.0 (filled columns) and pH 7.7 (open columns). Labels above the columns refer to the concentrations (mmol l–1) of Ca2+ (Ca), Mg2+ (Mg), D-lactate (D-l), and L-lactate (L-l). nh, native haemolymph; dHc, dialysed haemocyanin without added cofactor.

View larger version (16K): [in a new window]   Fig. 3. The effects of various ions on the cooperativity (n50) of Haliotis iris haemocyanin O2-binding at 15°C. Labels in the legend refer to the concentrations (mmol l–1) of Ca2+ (Ca), Mg2+ (Mg), D-lactate (D-l), and L-lactate (L-l). nh, native haemolymph; dHc, dialysed Hc without added cofactor.

View larger version (18K): [in a new window]   Fig. 4. Hill plot for dialysed haemocyanin of Haliotis iris measured in the absence (circles) and in the presence (triangles) of 50 mmol l–1 Mg2+, in 0.1 mol l–1 Bis-Tris buffer at pH 7.7 and 15°C. KT and KR are estimates of O2 association equilibrium constants (mmHg–1) for the low-affinity (T, tense) and high-affinity (R, relaxed) forms, respectively. S, fractional oxygen saturation.