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Carbon dioxide and pH affect sperm motility of white sturgeon (Acipenser transmontanus)

R. L. Ingermann*, M. Holcomb, M. L. Robinson and J. G. Cloud

Department of Biological Sciences and Center for Reproductive Biology, University of Idaho, Moscow, Idaho 83844-3051, USA



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  		Fig. 1. Effect of 2 h maintenance at various pH values on the ability of sperm to become motile upon addition to water. (A) Percentage motility of sperm from 6 males as a function of pH. (B) Same data set with motility expressed relative to that associated with the highest pH.

 


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  		Fig. 2. Motility versus time. Sperm maintained in low pH (6.91±0.02; filled squares) or high pH (9.72±0.03; open squares) SI buffer prior to water activation. N=5 for each data set. Earliest motility measurements were within 1 min of combining semen and SI buffers. If the t=0 data point is taken as 70±0% (the earliest motility value in high pH SI), all low pH motility values are significantly different from t=0. (Motility of sperm in high pH SI at 60 min was 70±0%, N=5.)

 


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  		Fig. 3. Relationship between PCO2 and pH of semen. Semen samples were equilibrated for 4 h at 10°C prior to pH analysis. 5-12 data points were collected from each of 6 males. (A) Curve represents the fourth order polynomial equation generated with all data. (B) Linear regression of pH as a function of the log of the PCO2. The line is given by the equation: pH=7.708-0.612 (logPCO2); r2=0.94 with 95% confidence limits for the slope being -0.653 to -0.571.

 


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  		Fig. 4. Buffering capacity of KT semen and seminal plasma. (A) Representative titration of semen (filled squares) and seminal plasma (open squares) with HCl at 10°C. (B) Buffering capacity of semen (filled squares) and seminal plasma (open squares) as a function of pH. Each value shown was calculated as the added HCl (in µmol HCl ml-1 semen or seminal fluid) divided by the resulting change in pH plotted against the median pH of each addition. Data represent 12-23 measurements from each of 6 males; curve represents the fourth order polynomial equation generated using all data points. (C) Buffering capacity of semen and seminal (Sem.) plasma determined by linear regression analysis of titration curves over the pH range 7.5-8.5 and 6.0-7.0; N=6. Buffering capacities of semen and seminal plasma were not different over either pH range. (Comparable data were obtained for UCD samples, N=4; data not shown.)

 

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© The Company of Biologists Ltd 2002