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Evidence for a mineralocorticoid-like receptor linked to branchial chloride cell proliferation in freshwater rainbow trout

Katherine A. Sloman^*, Patrick R. Desforges and Kathleen M. Gilmour

Department of Biology, Carleton University, Ottawa, ON, K1S 5B6 Canada

View larger version (92K): [in a new window]   Fig. 1. Representative light micrographs of trout gills showing filament and lamellae from untreated (A,B), RU486-treated (C,D) and spironolactone-treated (E,F) fish acclimated to dechlorinated city-of-Ottawa tapwater (control; A,C,E) or artificial softwater (B,D,F) for 7 days. Chloride cells, which are densely stained, have proliferated over the entire surface of the gill epithelium of the softwater-acclimated fish, with the exception of those fish treated with spironolactone (F). Accompanying the chloride cell proliferation is an increase in lamellar thickness and a reduction in interlamellar distances. Chloride cells of fish acclimated to control conditions (A,C,E) and of spironolactone-treated fish acclimated to softwater (F) are smaller and far less numerous.

View larger version (18K): [in a new window]   Fig. 2. The effect of acclimation condition (control versus softwater) and treatment (Untreated, Sham, RU486-treated and spironolactone-treated, Spiron.) on morphometric measurements for rainbow trout gills. (A) Chloride cell density, (B) lamellar thickness, (C) interlamellar distance. Values are means ± 1 S.E.M. (N=4 for each group). *A significant difference between control and softwater-acclimated fish within the same treatment group; a significant difference within an acclimation condition (control or softwater) from the untreated group (two-way ANOVA followed by Bonferroni’s post hoc multiple comparisons test, P<0.05).