Intestinal iron uptake in the European flounder (Platichthys flesus)
N. R. Bury1,*,
M. Grosell2,
C. M. Wood2,
C. Hogstrand1,
R. W. Wilson3,
J. C. Rankin4,
M. Busk4,
T. Lecklin4 and
F. B. Jensen4
1 Division of Health and Life Sciences, King’s College London, 150 Stamford Street, London SE1 9NN, UK,
2 Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada, L8S 4K1,
3 School of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter, Devon EX4 4PS, UK and
4 Institute of Biology, SDU, Odense University, Campusvej 55, Odense, Denmark
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Fig. 1. In vivo ferric (Fe3+) and ferrous (Fe2+) iron uptake (filled columns) from the posterior region of the intestine of the European flounder and the influence of the iron chelator desferrioxamine (DFO; open columns) on this uptake rate. Values are means + S.E.M. (N=5–6). An asterisk indicates a significant difference between the control iron (Fe3+ or Fe2+) uptake rate and the uptake rate in the presence of DFO, and a double dagger indicates a significant difference in uptake rate between the forms of iron, Fe3+ or Fe2+ (Student’s t-test, P<0.05).
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Fig. 2. In vivo ferrous (Fe2+) iron uptake rate in the different regions of the intestine of the European flounder. Values are means + S.E.M. (N=5–6). An asterisk indicates a significance difference between the iron uptake rates from the three regions (ANOVA followed by an LSD test, P<0.05).
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Fig. 5. In vitro ferrous iron (1 µmol l–1 Fe2+) absorption in the posterior region of the intestine of the European flounder at 1°C (filled columns) and 13°C (open columns). Values are means + S.E.M. (N=5–6).
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Fig. 6. In vitro rates of ferrous iron (Fe2+) absorption at 1 µmol l–1 in the three regions of the intestine of the European flounder, (A,D) anterior, (B,E) mid and (C,F) posterior, of control (filled circles and open columns) and anaemic (open circles and filled columns) fish. Values are means + S.E.M. (N=4–5). An asterisk indicates a significant difference in the rates of iron absorption (Student’s t-test, P<0.05). Linear regression analysis for iron absorption versus haematocrit shows r=–0.57, P=0.085 for the anterior region, r=0.10, P=0.63 for the mid region and r=–0.86, P=0.003 for the posterior region.
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Fig. 7. In vitro rate of ferrous iron (1 µmol l–1 Fe2+) absorption in the posterior region of the intestine of the European flounder at different pH values. The pH of the intestinal lumen changed over the experimental period; see text for details. Values are means + S.E.M. (N=5–6). There were no significant differences between rates of iron absorption.
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© The Company of Biologists Ltd 2001
, 
), mid (
, 
) and posterior (
, 
). Values are means ± S.E.M. (N=3–4). An asterisk indicates a significant difference between the absorption of Fe3+ and Fe2+ at a particular time (Student’s t-test, P<0.05), and values with differing letters indicate significant differences between Fe2+ absorption of the three regions at the 400 min incubation point (ANOVA followed by an LSD test, P<0.05). There are no significant difference between Fe2+ absorption of the three regions at the other time points.