QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rescan, P.-Y. Articles by Rallière, C. Search for Related Content PubMed PubMed Citation Articles by Rescan, P.-Y. Articles by Rallière, C. Social Bookmarking                         What's this?

Two myostatin genes are differentially expressed in myotomal muscles of the trout (Oncorhynchus mykiss)

Pierre-Yves Rescan^*, Ingrid Jutel and Cecile Rallière

SCRIBE-INRA, Campus de Beaulieu, 35042 Rennes, France

View larger version (73K): [in a new window]   Fig. 1. Nucleotide and deduced amino acid sequences of Tmyostatin 1 (A) and Tmyostatin 2 (B). The stop codon is indicated by a star. The sequences underlined correspond to the sense and antisense primers used to generate the intron-1-containing genomic fragment and for semi-quantitative RT-PCR. Accession numbers: AF273035 (Tmyostatin 1); AF273036 (Tmyostatin 2).

View larger version (81K): [in a new window]   Fig. 2. Comparison of the trout, zebrafish, chicken and mouse myostatin (mstn) proteins. The predicted proteolytic processing site is underlined in bold type, and the conserved cysteine residues in the C-terminal region found in all TGF-ß family members are marked with an asterisk. The shading indicates identity.

View larger version (8K): [in a new window]   Fig. 3. Nucleotide alignment of the 5' end of the first intron of Tmyostatin 1 and Tmyostatin 2 showing an additional insertion (in brackets) of a 509-nucleotide (nt) fragment within the first intron of the Tmyostatin 1 gene. Identity is indicated by a vertical line. The arrow indicates the exon/intron boundary. Accession numbers are AF278606 for intron 1 of Tmyostatin 1 and AF278607 for intron 1 of Tmyostatin 2.

View larger version (28K): [in a new window]   Fig. 4. Expression of Tmyostatin 1 (A) and Tmyostatin 2 (B) mRNA in trout adult tissues. Comparative RT-PCR of total RNA (1 µg) from, brain, heart, kidney, gills, intestine, testis, ovary, liver, fast muscle and slow muscle (lanes 1–10, respectively).

View larger version (31K): [in a new window]   Fig. 5. Expression of Tmyostatin 1 (A) and Tmyostatin 2 (B) mRNA in the myotomal muscle of developing rainbow trout embryos. Comparative RT-PCR of total RNA (1 µg) from the trunk of trout embryos at the eyed stage (lane 1), at hatching (lane 2) and in the myotomal musculature of a 5 g alevin (lane 3).

View larger version (68K): [in a new window]   Fig. 6. (A) Expression of Tmyostatin 1 (upper panel) and Tmyostatin 2 (lower panel) mRNA in myotomal muscle of male rainbow trout. Comparative RT-PCR of total RNA (1 µg) from fast-glycolytic (f) and slow-oxidative (s) muscles from five immature (lanes 1–10 from the left) and three spermiating (lanes 11–16 from the left) males. (B) Expression of Tmyostatin 1 (upper panel) and Tmyostatin 2 (lower panel) mRNA in myotomal muscle of female rainbow trout. Comparative RT-PCR of total RNA (1 µg) from fast-glycolytic (f) and slow-oxidative (s) muscles from five immature (lanes 1–10 from the left) and three spawning (lanes 11–16 from the left) females.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

Twitter