spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Summary Freely available
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Corrado, M. U. D.
Right arrow Articles by Falugi, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Corrado, M. U. D.
Right arrow Articles by Falugi, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Synthesis of the Signal Molecule Acetylcholine during the Developmental Cycle of PARAMECIUM PRIMAURELIA (Protista, Ciliophora) and its Possible Function in Conjugation

Maria Umberta Delmonte Corrado1,*, Huguette Politi1, Marzia Ognibene1, Cristiano Angelini2, Francesca Trielli1, Patrizia Ballarini3 and Carla Falugi2

1 Department for the Study of the Territory and its Resources, University of Genoa, Corso Europa 26, I-16132 Genoa, Italy,
2 Department of Experimental, Environmental, and Applied Biology, University of Genoa, Viale Benedetto XV 5, I-16132 Genoa, Italy and
3 Department of Molecular, Cellular, and Animal Biology, University of Camerino, Via Camerini 2, I-62032 Camerino, Italy



View larger version (56K):

[in a new window]
  		Fig. 1. Indirect immunofluorescence of choline acetyltransferase-related molecules analyzed by confocal laser scanning microscopy. Mating-competent mt I cell (Ai) and control (Aii). Mating-competent mt II cell (Bi) and control (Bii). Non-mating-competent cell (Ci) and control (Cii). Sections of an immature cell from the inside (D) to the surface (I). Scale bars, 20µm.

 


View larger version (81K):

[in a new window]
  		Fig. 2. Choline acetyltransferase activity, revealed as a dark precipitate, produced using the method of Burt (Burt, 1970). Mating-competent mt I cell (Ai) and control (Aii). Mating-competent mt II cell (Bi) and control (Bii). Immature cell (Ci) and control (Cii). Non-mating-competent cell (D). The control for the non-mating-competent cell is not shown because the complete absence of staining made it impossible to photograph. Scale bars, 20µm.

 


View larger version (38K):

[in a new window]
  		Fig. 3. Choline acetyltransferase (ChAT)-like molecules examined by immunoblot. Lane A, molecular mass standard marker; lane B, ChAT from human placenta; lanes C and D, membrane lysates from mating-competent mt I and mt II cells, respectively; lanes E and F, membrane lysates from non-mating-competent and immature cells, respectively; lanes G and H, cytoplasmic fractions from mating-competent mt I and mt II cells, respectively; lanes I and L, cytoplasmic fractions from non-mating-competent and immature cells, respectively.

 

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



© The Company of Biologists Ltd 2001