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Journal of Experimental Biology, Vol 98, Issue 1 429-438, Copyright © 1982 by Company of Biologists


JOURNAL ARTICLES

Protein and glycoprotein antifreezes in the intestinal fluid of polar fishes

SM O'Grady, JC Ellory and AL DeVries

Measurements of ion concentrations, freezing points and melting points of intestinal fluid were made for several Antarctic fishes and two North Atlantic species. These measurements indicated that plasma and intestinal fluid are nearly isosmotic. Freezing points of intestinal fluid were approximately 0.9 degrees C below the melting points, suggesting the presence of glyco-protein antifreeze within the intestinal fluid of the Antarctic fishes. Polyacrylamide gel electrophoresis and specific immunoprecipitation with glyco-protein antifreeze antibody confirmed the presence of appreciable quantities of antifreeze and showed that the major antifreeze fractions present in the intestinal fluid are low molecular weight glycopeptides.


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© The Company of Biologists Ltd 1982