QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by TAGHERT, P. H. Articles by TRUMAN, J. W. Search for Related Content PubMed Articles by TAGHERT, P. H. Articles by TRUMAN, J. W.

Journal of Experimental Biology 98,373-383 (1982)
Published by Company of Biologists 1982

The Distribution and Molecular Characteristics of the Tanning Hormone, Bursicon, in the Tobacco Hornworm Manduca Sexta

PAUL H. TAGHERT ¹ and JAMES W. TRUMAN ²

¹ Department of Zoology, University of Washington Seattle, WA 98195, U.S.A
² Department of Zoology, University of Washington Seattle, WA 98195, U.S.A.

To whom correspondence should be addressed. Present address: Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.

The distribution and molecular properties of the tanning hormone bursicon were studied using an isolated wing biological assay. Wing cuticle tanning activity was measured in saline homogenates of the central nervous system and selected muscles. Activity was detected in all ganglia of the nervous system in both the prepupal and pharate adult developmental stages. In both stages, this activity was predominantly located in the abdominal portion of the nervous system. The titres in all ganglia, except the suboesophageal, increased during metamorphosis. Of the various non-neural tissues examined, only the closer muscle of the spiracle contained detectable levels of activity.

All activity in the nervous tissue was sensitive to proteolytic digestion; it could not be mimicked by any of the 17 putative transmitter and/or hormonal substances tested. Partial purification of the tanning activity indicated an apparent molecular weight of 20-30 K.

The partially purified material (from gel filtration) resembled the hormone bursicon in the following three ways: its titre in the tissue declined following normal bursicon release; it could be recovered from the haemolymph during normal bursicon release; it could be released from isolated nervous tissue following high potassium stimulation in a calcium-dependent manner.

It was concluded that the hormone bursicon represents most if not all the tanning activity present in the central nervous system, that it is widely distributed in that tissue and that it is present as a peptide (or class of peptides) with homogeneous size and charge.

This article has been cited by other articles:

				H. Luan, W. C. Lemon, N. C. Peabody, J. B. Pohl, P. K. Zelensky, D. Wang, M. N. Nitabach, T. C. Holmes, and B. H. White Functional Dissection of a Neuronal Network Required for Cuticle Tanning and Wing Expansion in Drosophila J. Neurosci., January 11, 2006; 26(2): 573 - 584. [Abstract] [Full Text] [PDF]

				C.-W. Luo, E. M. Dewey, S. Sudo, J. Ewer, S. Y. Hsu, H.-W. Honegger, and A. J. W. Hsueh Bursicon, the insect cuticle-hardening hormone, is a heterodimeric cystine knot protein that activates G protein-coupled receptor LGR2 PNAS, February 22, 2005; 102(8): 2820 - 2825. [Abstract] [Full Text] [PDF]

				A Schmid, A Chiba, and C. Doe Clonal analysis of Drosophila embryonic neuroblasts: neural cell types, axon projections and muscle targets Development, January 11, 1999; 126(21): 4653 - 4689. [Abstract] [PDF]

				N. Tublitz and J. Truman Intracellular stimulation of an identified neuron evokes cardioacceleratory peptide release Science, May 24, 1985; 228(4702): 1013 - 1015. [Abstract] [PDF]