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Journal of Experimental Biology 96,221-237 (1982)
Published by Company of Biologists 1982

The Influence of Experimental Anaemia on Blood Acid-Base Regulation In Vivo and In Vitro in the Starry Flounder (Platichthys Stellatus) and the Rainbow Trout (Salmo Gairdneri)

CHRIS M. WOOD ¹, D. G. McDONALD ¹, and B. R. McMAHON ²

¹ Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1
² Department of Biology, University of Calgary, Calgary, Alberta, Canada T2N 1N4

Severe experimental anaemia caused a rise in P_coco2 and an associated fall in pH (respiratory acidosis) in arterial and venous blood of both flounder and trout in vivo. In some trout, but not in flounder, there was also a rise in blood lactate, indicating metabolic acidosis. In vitro, blood buffer capacities declined with haematocrit, a factor which contributed to the extent of the acidoses in vivo. However, haematocrit did not influence the pK¹ of the plasma HCO₃^-/H₂CO₃ system or the actual measurement of blood pH. The Donnan ratio for HCO₃^- varied linearly with pH over the range 7.0–7.6, indicating a passive distribution of HCO₃^- across the trout erythrocyte. The present data, together with other recent results, indicate that the teleost red blood cell does play a role in plasma HCO₃^- dehydration and CO₂ excretion, and therefore opposes the theory of Haswell & Randall (1978) that the erythrocyte is functionally impermeable to HCO₃^-.

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