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Journal of Experimental Biology 92,289-303 (1981)
Published by Company of Biologists 1981

Branchial Ionic Uptake and Acid-Base Regulation in the Rainbow Trout, Salmo Gairdneri

S. F. PERRY ¹, M. S. HASWELL ², D. J. RANDALL ¹, and A. P. FARRELL ³

¹ The University of British Columbia, Department of Zoology, #2354-6270 University Blvd., Vancouver, B.C., Canada V6T 2A9
² Colorado State University, Department of Zoology and Entomology, Fort Collins, Colorado, U.S.A. 80523
³ Cardiovascular Research Laboratory, University of Southern California Medical Center, 1200 North State Street, Box 1800, Los Angeles, California, U.S.A. 90033

1. Amiloride (10^-4 M) inhibited sodium uptake in rainbow trout by 78% and was associated with a pronounced acidosis and decreases in both plasma total CO₂ (T_coco2)^* and [HCO₃^-].

2. 4-acetamido-4'-iso-thiocyanatostilbene-2,2' disulphonic acid (SITS) (10^-4M) in the bathing medium inhibited chloride uptake by 66% and following 6 h a significant decrease in plasma [H⁺] and significant increases in T_COCO2 and [HCO₃^-] were observed.

3. Inhibition of chloride uptake (50%) with external sodium bicarbonate (12 mM) resulted in a more rapid and pronounced alkalosis than did SITS inhibition.

4. Hypercapnic acidosis had no significant effect on the rates of branchial sodium and chloride uptake.

5. Increasing the concentration of sodium in the bathing water resulted in a less pronounced acidosis and a more rapid pH recovery during hypercapnia.

6. These results are discussed with reference to the gill as an acid-base regulating structure. These findings are consistent with a gill model previously presented by Haswell, Randall & Perry (1980).

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