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Journal of Experimental Biology 78,255-264 (1979)
Published by Company of Biologists 1979

Temperature-Related Changes in the Erythrocytic Carbonic Anhydrase (Acetazolamide-Sensitive Esterase) Activity of Goldfish, Carassius Auratus

ARTHUR H. HOUSTON ¹ and KAREN M. MEAROW ¹

¹ Department of Biological Sciences, Brock University, St Catharines, Ontario, Canada, L2S 3A1

1. Carbonic anhydrase activity in ‘membrane’ and ‘cytosol’ fractions of goldfish erythrocytes was assayed by the p-nitrophenyl acetate procedure following thermal acclimation.

2. The thermal sensitivity of ‘membrane’-associated activity was apparently unaltered by acclimation. ‘Cytosol’ activity in warm-acclimated specimens was somewhat more thermosensitive than that of animals maintained at low temperature.

3. Significant increases in specific activity, and activity per unit volume of packed cells and blood were observed at higher temperatures when assays were conducted at the temperatures at which the system actually functions in the fish. By contrast, when determinations were carried out at a standard temperature (41 °C) corresponding to the upper incipient lethal for this species, activity was either unaffected, or declined as acclimation temperatures increased.

4. Changes in carbonic anhydrase activity following acclimation are consistent with the hypothesis that this system is implicated in the maintenance of stable plasma chloride levels, and the suggestion that alterations in red cell chloride levels with temperature are, in part at least, attributable to concomitant variations in enzyme activity.

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