spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LITTLE, C.
Right arrow Search for Related Content
PubMed
Right arrow Articles by LITTLE, C.
Journal of Experimental Biology 61,667-675 (1974)
Published by Company of Biologists 1974

A Method for Determining Total Carbon Dioxide in Nanolitre Volumes of Liquid

COLIN LITTLE 1

1 Department of Zoology, University of Bristol, Bristol BS8 1UG

1. A microdiffusion method is described by which total carbon dioxide can be determined in 2 nl samples of liquid. The standard deviation for 0-30 mM/l HCO3- is approximately 1 mM/l.

2. The volume of sample can be reduced to o·8 nl, but the S.D. then rises to 1·6 mM/l. With volumes of 0·4 nl, the S.D. is 2·7 mM/l. It is not possible, without modification, to use volumes smaller than about 0·4 nl.

3. In samples where the HCO3- concentration is low, accuracy can be increased by using larger volumes: 10 mM/l HCO3- can be determined in 6 nl samples with a standard deviation of 0·5 mM/l.

Submitted on June 8, 1974






© The Company of Biologists Ltd 1974