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Journal of Experimental Biology 56,323-333 (1972)
Published by Company of Biologists 1972

The Effects of 5-Hydroxytryptamine and Cyclic Amp on the Potential Profile across Isolated Salivary Glands

WILLIAM T. PRINCE 1 and MICHAEL J. BERRIDGE 2

1 A.R.C. Unit of Invertebrate Chemistry and Physiology, Department of Zoology, Downing Street, Cambridge
2 A.R.C. Unit of Invertebrate Chemistry and Physiology, Department of Pharmacology, Hills Road, Cambridge

1. The sites of the transepithelial potential changes produced by 5-HT and cyclic AMP in the salivary gland of the blowfly (Calliphora) have been investigated.

2. Microelectrodes recorded a two-step potential profile across this epithelium. The cell was negative to both the bathing medium and the saliva.

3. The basal membrane potential was 44·0±0·2 mV and was affected very little by application of either 5-HT or cyclic AMP.

4. The apical membrane was depolarized by 5-HT and in contrast was hyperpolarized by cyclic AMP.

5. On the basis of these results it is concluded that 5-HT has two actions: (a) to stimulate a potassium pump on the apical membrane using cyclic AMP as an intermediary, (b) to increase chloride movement by a mechanism not directly involving cyclic AMP.

Submitted on September 10, 1971




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© The Company of Biologists Ltd 1972