QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by CIVIL, G. W. Articles by THOMPSON, T. E. Search for Related Content PubMed Articles by CIVIL, G. W. Articles by THOMPSON, T. E.

Journal of Experimental Biology 56,239-247 (1972)
Published by Company of Biologists 1972

Experiments with the Isolated Heart of the Gastropod Helix Pomatia in an Artificial Pericardium

G. W. CIVIL ¹ and T. E. THOMPSON ¹

¹ Zoology Department, University of Bristol, U.K.

1. Isolated hearts of Helix pomatia could be maintained for 3-4 days at 19 °C in physiological saline. Co-ordination was soon lost, but irregular twitches could be observed for up to 115 h.

2. A perfusion apparatus was designed which supplied a simulated venous return pressure of 8 cm saline and enabled in vitro survival of pericardium-free hearts for up to 2 days at 15 °C. Cessation of perfusion led immediately to a reversible stoppage of heart-beat.

3. An artificial pericardium apparatus (APA) allowed the role of the pericardium to be studied. In the APA measurable translocation of fluid was effected by the heart, even when the simulated venous return pressure was negative.

4. If the APA was transformed into an open system by opening up a simulated reno-pericardial canal, the effectiveness of the heart was greatly reduced.

5. In the APA the greater the dilation of the heart (in consequence of decreased volume of pericardial fluid) the greater was the stroke volume for a given simulated venous return pressure.

6. Results obtained with the APA give support to the theory that in the gastropod heart the filling of the auricle is hydrodynamically coupled (through the pericardial fluid) to the emptying of the ventricle.