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Journal of Experimental Biology 56,139-153 (1972)
Published by Company of Biologists 1972

Transepithelial Potential Changes During Stimulation of Isolated Salivary Glands with 5-Hydroxytryptamine and Cyclic Amp

MICHAEL J. BERRIDGE 1 and WILLIAM T. PRINCE 1

1 Unit of Invertebrate Chemistry and Physiology, Department of Zoology, Downing Street, Cambridge, and Department of Pharmacology, Hills Road, Cambridge

1. The role of cyclic AMP in mediating the action of 5-HT on salivary glands has been studied by measuring transepithelial potentials.

2. The lumen of unstimulated glands is 4 mV positive but becomes 12 mV negative after treatment with 5-HT (10-8 M). Both the potential and the secretory responses to 5-HT are dose-dependent over the same concentration range.

3. The electrical response of salivary glands to cyclic AMP is qualitatively different to that of 5-HT; instead of going negative the potential goes more positive.

4. An increase in positive potential is also observed after treatment with theophylline (10-2 M), or when glands are stimulated with 5-HT in a chloride-free saline.

5. These results are consistent with the idea that 5-HT has two actions. One is to stimulate the enzyme adenyl cyclase to synthesize cyclic AMP, which, in turn, stimulates cation transport. The other is to increase anion transport by a mechanism which is independent of cyclic AMP.

Submitted on May 21, 1971




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© The Company of Biologists Ltd 1972