QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by STONER, L. C. Articles by DUNHAM, P. B. Search for Related Content PubMed PubMed Citation Articles by STONER, L. C. Articles by DUNHAM, P. B.

Journal of Experimental Biology 53,391-399 (1970)
Published by Company of Biologists 1970

Regulation of Cellular Osmolarity and Volume in Tetrahymena

LARRY C. STONER ¹ and PHILIP B. DUNHAM ¹

¹ Department of Zoology, Syracuse University, Syracuse, New York, U.S.A.

1. Tetrahymena pyriformis are hyperosmotic to external media of osmolarities from 2 to 171 m-osmole/l. The intracellular osmolarity, determined by freezing-point depression, is 111 m-osmole/kg cells in dilute media, and increases linearly with increasing external osmolarities.

2. Over 80% of the intracellular osmolarity can be attributed to the concentration of sodium, potassium, chloride and the free amino acids.

3. In response to an increase in the external osmolarity, Tetrahymena regulates its intracellular osmolarity by increasing the concentrations of free amino acids.

4. The regulation of cellular volume under conditions of osmotic stress is achieved by an increase in the amount of osmotically active solutes and the regulation of the rate of elimination of fluid by the contractile vacuole.

This article has been cited by other articles:

				M. Iwamoto, K. Sugino, R. D. Allen, and Y. Naitoh Cell volume control in Paramecium: factors that activate the control mechanisms J. Exp. Biol., February 1, 2005; 208(3): 523 - 537. [Abstract] [Full Text] [PDF]

				B. Becker and A. Hickisch Inhibition of Contractile Vacuole Function by Brefeldin A Plant Cell Physiol., January 15, 2005; 46(1): 201 - 212. [Abstract] [Full Text] [PDF]

				C. Stock, H. K. Gronlien, R. D. Allen, and Y. Naitoh Osmoregulation in Paramecium: in situ ion gradients permit water to cascade through the cytosol to the contractile vacuole J. Cell Sci., January 6, 2002; 115(11): 2339 - 2348. [Abstract] [Full Text] [PDF]

				C Stock, R. Allen, and Y Naitoh How external osmolarity affects the activity of the contractile vacuole complex, the cytosolic osmolarity and the water permeability of the plasma membrane in Paramecium multimicronucleatum J. Exp. Biol., January 1, 2001; 204(2): 291 - 304. [Abstract] [PDF]

				E Orias, E. Hamilton, and M Flacks Osmotic shock prevents nuclear exchange and produces whole-genome homozygotes in conjugating Tetrahymena Science, February 16, 1979; 203(4381): 660 - 663. [Abstract] [PDF]