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Journal of Experimental Biology 36,177-190 (1959)
Published by Company of Biologists 1959

A New Tonometric Method for the Determination of Dissolved Oxygen and Carbon Dioxide in Small Samples

J. D. JONES 1

1 Department of Zoology, University of Sheffield

1. A microtonometric method is described whereby tensions of carbon dioxide, oxygen and nitrogen can be determined in fluid samples of 0.3 ml. volume or less, each determination taking 20-25 min.

2. Replicate determinations of the tensions of carbon dioxide, oxygen and nitrogen give maximum coefficients of variability of 3.0, 2.2 and 1.2%, respectively.

3. A comparison of the present method with a micro-Winkler method for the determination of dissolved oxygen shows a close agreement; the mean percentage difference being 3.0.

Submitted on June 26, 1958






© The Company of Biologists Ltd 1959