QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by EASTY, G. C. Articles by AMBROSE, E. J. Search for Related Content PubMed Articles by EASTY, G. C. Articles by AMBROSE, E. J.

Journal of Experimental Biology 34,60-70 (1957)
Published by Company of Biologists 1957

An Interferometric Method of Quantitative Analysis of Antigen-Antibody Reactions in Gels

G. C. EASTY ¹ and E. J. AMBROSE ¹

¹ Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, London, S.W.3

1. A sensitive interferometric method of observing antigen-antibody complexes formed by diffusion of the reactants through gels in suitable diffusion cells is described.

2. Bands of antigen-antibody complex, if not too dense, are observed as peaks in the interference fringes, the areas under the peaks being proportional to the quantities present in each band.

3. The method may be made quantitative by measurement of the areas under the peaks of bands in which precipitation has not progressed too far, and by dissolving the bands of dense precipitate by diffusion of a suitable reagent to give peaks in the fringes.

4. An equation expressing the quantity of precipitate present in a band has been derived.