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Journal of Experimental Biology 32,822-829 (1955)
Published by Company of Biologists 1955

ELECTROMETRIC TITRATION OF CHLORIDE IN SMALL VOLUMES

J. A. RAMSAY ¹, R. H. J. BROWN ¹, and P. C. CROGHAN ¹

¹ Zoological Laboratory, University of Cambridge

1. Two methods of titrating chloride with Ag⁺ ion using the potentiometric end-point are described.

2. The first method is conventional in that silver nitrate is added from a burette. It deals with volumes down to 0.2µl. and can measure 1µg. of chloride with an error of < ± 1% (standard deviation).

3. According to the second method Ag⁺ ion is added by passing a current through a silver electrode in series with a condenser. The charge developed on the condenser is a measure of the amount of chloride titrated. This method deals with volumes down to 0.5 x 10^-3µl. and can measure 10^-4µg. of chloride with an error of < ± 1% (standard deviation).

4. As far as is known these methods are not susceptible to interference from other substances likely to be present in biological fluids.

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