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The Fertilization Reaction in the Sea-Urchin : The Induction of Polyspermy by Nicotine
1 The Zoological Station, Naples, and the Department of Zoology, University of Cambridge
1. Treatment of unfertilized sea-urchin eggs with sea water containing nicotine is known to induce polyspermy when the eggs are subsequently inseminated with homologous spermatozoa, at densities which cause a very small amount of polyspermy in untreated eggs.
2. If nicotine were to increase the speeds at which sea-urchin spermatozoa swim, the chances of fertilization, and therefore of polyspermy, might be increased. Nicotine does not increase sperm speeds; in addition, it causes a sharp reduction in the O2 uptake of these spermatozoa.
3. The only other ways in which nicotine could induce polyspermy are by altering the egg surface in such a way that the probability of fertilization is increased by a factor of about twenty; or by lengthening the conduction time of the block to polyspermy. Experiments described in this paper show that the first explanation is untenable and therefore that the second is the correct one. It is concluded that nicotine abolishes the fast incomplete block to polyspermy and that over-exposure to this substance probably abolishes the block to polyspermy altogether.
4. Polyspermic eggs divide sooner than monospermic ones.
5. When, as in these experiments, eggs are allowed to interact with spermatozoa for known times, and then functionally separated by immersion in hypotonic sea water, some eggs, presumably those which sustain a successful sperm-egg collision at the end of the interaction time, are activated but not fertilized by the spermatozoon, as in the pseudogamous nematodes. Cleavage does not occur though the egg nucleus swells.
6. Previous results in the same field and observations by other workers are discussed.
Submitted on June 9, 1952
