QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by CLELAND, K. W. Articles by ROTHSCHILD, L. Search for Related Content PubMed Articles by CLELAND, K. W. Articles by ROTHSCHILD, L.

Journal of Experimental Biology 29,416-428 (1952)
Published by Company of Biologists 1952

The Metabolism of the Sea-Urchin Egg : Oxidation of Carbohydrate

K. W. CLELAND ¹ and LORD ROTHSCHILD ²

¹ Department of Zoology, University of Cambridge, and the Marine Station, Millport; University of Sydney, Department of Histology and Embryology
² Department of Zoology, University of Cambridge, and the Marine Station, Millport

1. The endogenous O₂ uptake of sea-urchin egg homogenates (Echinus esculentus) was inhibited by malonate, fluoride, iodoacetate, and fluoracetate.

2. The addition of the tricarboxylic acid cycle intermediates, citrate, -ketoglutarate, succinate, fumarate, malate, and glutamate stimulated O₂ uptake.

3. The efficiency of the pyruvate-oxidizing mechanism varied in different preparations, but in some, added pyruvate was completely oxidized.

4. (1)-(3) strongly suggest the presence of a tricarboxylic acid cycle.

5. Glycogen, glucose and fructose stimulated O₂ uptake. The oxidation of fructose was stimulated by DPN and inhibited by fluoride and iodoacetate.

6. Glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate and fructose-1:6 diphosphate stimulated O₂ uptake.

7. Phosphogluconate also stimulated O₂ uptake, which suggests that the enzymes of the hexose monophosphate shunt system may be present.

8. The experiments described in this paper show that the principal pathway by which carbohydrate is broken down is the normal glycolytic one.

This article has been cited by other articles:

				C. S. HAMMEN and P. J. OSBORNE Carbon Dioxide Fixation in Marine Invertebrates: A Survey of Major Phyla Science, November 20, 1959; 130(3386): 1409 - 1410. [Abstract] [PDF]

				C. M. MONTGOMERY and J. W. BAMBERGER Action of Parapyruvate on Early Development of Strongylocentrotus Purpuratus Science, November 18, 1955; 122(3177): 967 - 968. [PDF]