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Journal of Experimental Biology 27,407-419 (1950)
Published by Company of Biologists 1950


The Determination of Sodium in Small Volumes of Fluid by Flame Photometry

J. A. RAMSAY 1

1 Zoological Laboratory, University of Cambridge

1. A method of integrative flame photometry is described, by which the amount of sodium in biological fluids can be determined, using samples of the order of 10-3 cu.mm.

2. With pure solutions of sodium chloride, of concentration from 0.05 to 2.0 %, the average error of the method is about 3 % of the concentration.

3. When other substances are present systematic errors of up to 7.7 % have been recorded.

4. The possibilities of reducing these systematic errors and of extending the method to deal with other elements are discussed.

Submitted on May 16, 1950




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