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Journal of Experimental Biology 27,253-256 (1950)
Published by Company of Biologists 1950

Studies on Haemovanadin. III. Some Physiological Properties of Haemovanadin, The Vanadium Compound of the Blood of Phallusia Mamillata Cuv

LUIGI CALIFANO 1 and ENZO BOERI 1

1 Stazione Zoologica di Napoli

A method for obtaining stable solutions of haemovanadin from the blood of Phallusia mamillata Cuv. is described. It is essential that all operations be carried out at pH 2.4.

Native haemovanadin reduces cytochrome c both under aerobic and anaerobic conditions. Solutions of V2O2 also reduce cytochrome c. The reduction takes place with a stoichiometric ratio V++: Fe+++ of one to one.

The sperm and ripe eggs of the animal contain cytochrome c. The pigment was not found in other tissues.

Cyanide does not inhibit the reducing power of haemovanadin towards cytochrome c or methylene blue.

Note:

A preliminary report of this work was presented at the First International Congress of Biochemistry, Cambridge, August 1949.

Submitted on February 28, 1950






© The Company of Biologists Ltd 1950