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Journal of Experimental Biology 26,380-387 (1950)
Published by Company of Biologists 1950

The Nutritional Requirements of Drosophila Melanogaster

MICHAEL BEGG 1 and FORBES W. ROBERTSON 1

1 Zoology Department, Marischal College, University of Aberdeen, and Animal Breeding and Genetics Research Organization, Institute of Animal Genetics Edinburgh

1. Methods are described for the aseptic culture of Drosophila.

2. All the factors necessary for normal development can be extracted from yeast water-soluble form.

3. A chemically defined medium sufficient for almost normal growth is described and includes: casein (and gelatin) as a source of amino-acids, dextrose, cholesterol, ergosterol, yeast nucleic acid, inositol, biotin, aneurin hydrochloride, riboflavine, nicotinic acid, pyridoxin hydrochloride, Ca-pantothenate, choline chloride, thymine and folic acid.

4. For completely normal development an alkali-soluble fraction must be added. This fraction shows nucleoprotein-like reaction, but it is neither arginine nor nucleic acid.

5. Folic acid is essential for pupation.

6. Attention is drawn to the need for caution in gene-action studies with synthetic media which do not give completely normal development even in the wild type.

Submitted on July 18, 1949




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