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First published online September 11, 2009
Journal of Experimental Biology 212, 3156-3163 (2009)
Published by The Company of Biologists 2009
doi: 10.1242/jeb.029249
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Oxygen tension correlates with regional blood flow in obstructed rat kidney

Anja M. Jensen1,2,3,*, Rikke Nørregaard1,2, Sukru Oguzkan Topcu1,2, Jørgen Frøkiær1,2,3 and Michael Pedersen2,4

1 The Water and Salt Research Center, Aarhus University Hospital, Skejby, DK 8200, Aarhus N, Denmark
2 Institute of Clinical Medicine, Aarhus University Hospital, Skejby, DK 8200, Aarhus N, Denmark
3 Department of Clinical Physiology and Nuclear Medicine, Aarhus University Hospital, Skejby, DK 8200, Aarhus N, Denmark
4 MR Research Center, Aarhus University Hospital, Skejby, DK 8200, Aarhus N, Denmark

* Author for correspondence (anja.jensen{at}ki.au.dk)

Accepted 29 June 2009

As renal tissue oxygen tension (PO2) is determined by the balance between oxygen supply and consumption, direct tissue PO2 measurements are essential when evaluating the presence of hypoxia. The present study aimed at evaluating invasively and continuously the renal medullary and cortical tissue PO2 by novel fibre-optic probes in rats subjected to acute unilateral ureteral obstruction (AUUO). In parallel, regional blood flow measurements were obtained by MRI to investigate the relationship between regional blood flow and tissue oxygen tension. The abundance of transport proteins was determined by immunoblotting. In the obstructed kidney, AUUO caused a prompt decrease in medullary tissue PO2 to 60% of baseline level whereas cortical tissue PO2 was unchanged. By contrast, tissue PO2 slightly increased in the non-obstructed kidney. These changes developed during the first 30 min after AUUO and persisted for the 3 h observation period. Medullary blood flow declined 1.5–2 h after induction of AUUO to 61% of baseline level in the obstructed kidney. By contrast, cortical blood flow increased to 108% of baseline level in the non-obstructed kidney. Finally, the abundance of phosphorylated aquaporin 2 decreased significantly in the obstructed kidney medulla, but increased in the obstructed kidney cortex. The Na+/K+-ATPase abundance increased in the obstructed kidney medulla whereas the Na+/K+/2Cl– co-transporter abundance remained unchanged in the obstructed kidney. In conclusion, measurements of regional blood flow reflect tissue PO2 changes during AUUO suggesting that reduced regional blood flow is a predictor of local hypoxia. Furthermore, the abundance of major transport protein is independent of tissue PO2.

Key words: kidney, hypoxia, perfusion


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