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First published online September 11, 2009
Journal of Experimental Biology 212, 3132-3141 (2009)
Published by The Company of Biologists 2009
doi: 10.1242/jeb.031179
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Metabolic function in Drosophila melanogaster in response to hypoxia and pure oxygen

Wayne A. Van Voorhies

Molecular Biology Program, MSC 3MLS, New Mexico State University, Las Cruces, NM 88003, USA

wvanvoor{at}nmsu.edu

Accepted 18 June 2009

This study examined the metabolic response of Drosophila melanogaster exposed to O2 concentrations ranging from 0 to 21% and at 100%. The metabolic rate of flies exposed to graded hypoxia remained nearly constant as O2 tensions were reduced from normoxia to ~3 kPa. There was a rapid, approximately linear reduction in fly metabolic rate at PO2s between 3 and 0.5 kPa. The reduction in metabolic rate was especially pronounced at PO2 levels <0.5 kPa, and at a PO2 of 0.1 kPa fly metabolic rate was reduced ~10-fold relative to normoxic levels. The metabolic rate of flies exposed to anoxia and then returned to normoxia recovered to pre-anoxic levels within 30 min with no apparent payment of a hypoxia-induced oxygen debt. Flies tolerated exposure to hypoxia and/or anoxia for 40 min with nearly 100% survival. Fly mortality increased rapidly after 2 h of anoxia and >16 h exposure was uniformly lethal. Flies exposed to pure O2 for 24 h showed no apparent alteration of metabolic rate, even though such O2 tensions should damage respiratory enzymes critical to mitochondria function. Within a few hours the metabolic rate of flies recovering from exposure to repeated short bouts of anoxia was the same as flies exposed to a single anoxia exposure.

Key words: metabolic rate, reperfusion injury, critical oxygen tension, hypoxic response, hyperoxia


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© The Company of Biologists Ltd 2009