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First published online May 29, 2009
Journal of Experimental Biology 212, 1840-1848 (2009)
Published by The Company of Biologists 2009
doi: 10.1242/jeb.026278

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Plasmatocyte-spreading peptide (PSP) plays a central role in insect cellular immune defenses against bacterial infection

I. Eleftherianos^1,*, M. Xu¹, H. Yadi¹, R. H. ffrench-Constant² and S. E. Reynolds¹

¹ Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK
² School of Biological Sciences, University of Exeter, Cornwall Campus, Penryn, Cornwall TR10 9EZ, UK

^* Author for correspondence (Ioannis.Eleftherianos{at}ibmc.u-strasbg.fr)

Accepted 22 March 2009

Insect hemocytes (blood cells) are a central part of the insect's cellular response to bacterial pathogens, and these specialist cells can both recognize and engulf bacteria. During this process, hemocytes undergo poorly characterized changes in adhesiveness. Previously, a peptide termed plasmatocyte-spreading peptide (PSP), which induces the adhesion and spreading of plasmatocytes on foreign surfaces, has been identified in lepidopteran insects. Here, we investigate the function of this peptide in the moth Manduca sexta using RNA interference (RNAi) to prevent expression of the precursor protein proPSP. We show that infection with the insect-specific bacterial pathogen Photorhabdus luminescens and non-pathogenic Escherichia coli induces proPSP mRNA transcription in the insect fat body but not in hemocytes; subsequently, proPSP protein can be detected in cell-free hemolymph. We used RNAi to silence this upregulation of proPSP and found that the knock-down insects succumbed faster to infection with P. luminescens, but not E. coli. RNAi-treated insects infected with E. coli showed a reduction in the number of circulating hemocytes and higher bacterial growth in hemolymph as well as a reduction in overall cellular immune function compared with infected controls. Interestingly, RNAi-mediated depletion of proPSP adversely affected the formation of melanotic nodules but had no additional effect on other cellular responses when insects were infected with P. luminescens, indicating that this pathogen employs mechanisms that suppress key cellular immune functions in M. sexta. Our results provide evidence for the central role of PSP in M. sexta cellular defenses against bacterial infections.

Key words: insect immunity, cellular response, plasmatocyte-spreading peptide, RNAi interference, Manduca sexta, Photorhabdus luminescens

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