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First published online September 5, 2008
Journal of Experimental Biology 211, 3020-3027 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.016360
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Natural odor ligands for olfactory receptor neurons of the female mosquito Aedes aegypti: use of gas chromatography-linked single sensillum recordings

Majid Ghaninia1,2,*, Mattias Larsson1, Bill S. Hansson1,3 and Rickard Ignell1

1 SLU, Department of Plant Protection Biology, 230 53 Alnarp, Sweden
2 Department of Plant Protection, College of Agriculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Max Plank Institute for Chemical Ecology, Department of Evolutionary Neuroethology, DE-07745 Jena, Germany

* Author for correspondence (e-mail: majid.ghaninia{at}vv.slu.se)

Accepted 16 July 2008

Female Aedes aegypti are vectors of dengue and yellow fever. Odor volatiles are the predominant cues that drive the host-seeking behavior of Ae. aegypti. Odorant molecules are detected and discriminated by olfactory receptor neurons (ORNs) housed in sensory hairs, sensilla, located on the antennae and maxillary palps. In a previous study, we used odor volatiles that are behaviorally and/or electrophysiologically active for Ae. aegypti and other mosquito species to show that antennal ORNs of female Ae. aegypti are divided into functionally different classes. In the present study, we have, for the first time, conducted gas chromatography-coupled single sensillum recordings (GC–SSR) from antennal trichoid and intermediate sensilla of female Ae. aegypti in order to screen for additional putative host attractants and repellents. We used headspace collections from biologically relevant sources, such as different human body parts (including feet, trunk regions and armpit), as well as a plant species used as a mosquito repellent, Nepeta faassenii. We found that a number of ORN types strongly responded to one or more of the biological extracts. GC–SSR recordings revealed several active components, which were subsequently identified through GC-linked mass spectrometry (GC–MS). Electrophysiologically active volatiles from human skin included heptanal, octanal, nonanal and decanal.

Key words: Aedes aegypti, biologically active volatiles, electrophysiology, olfactory receptor neurons


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