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First published online June 27, 2008
Journal of Experimental Biology 211, 2196-2204 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.018606
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Thermal biology of the deep-sea vent annelid Paralvinella grasslei: in vivo studies

Delphine Cottin1,2, Juliette Ravaux1,2,*, Nelly Léger1,2, Sébastien Halary1,2, Jean-Yves Toullec3,4, Pierre-Marie Sarradin5, Françoise Gaill1,2 and Bruce Shillito1,2

1 UPMC Université Paris 6, UMR 7138, `Systématique, Adaptation et Evolution', F-75005 Paris, France
2 CNRS UMR 7138, `Systématique, Adaptation et Evolution', F-75005, Paris, France
3 UPMC Université Paris 6, FRE 2852 `Protéines: Biochimie Structurale et Fonctionnelle', F-75005 Paris, France
4 CNRS FRE 2852 `Protéines: Biochimie Structurale et Fonctionnelle', F-75005 Paris, France
5 DEEP/Laboratoire Environnement Profond, Centre IFREMER de Brest, bp 70, 29280 Plouzané, Cedex, France

* Author for correspondence (e-mail: juliette.ravaux{at}snv.jussieu.fr)

Accepted 1 May 2008

The annelid Paralvinella grasslei is a deep-sea vent endemic species that colonizes the wall of active chimneys. We report here the first data on its thermal biology based on in vivo experiments in pressurized aquaria. Our results demonstrate that P. grasslei survives a 30 min exposure at 30°C, and suggest that the upper thermal limit of this species is slightly above this temperature. The first signs of stress were noticed at 30°C, such as a significant increase in the animal's activity and the expression of HSP70 stress proteins. A preliminary investigation of the kinetics of stress protein expression surprisingly showed high levels of HSP70 proteins as late as 3.5 h after the heat shock. Finally, we provide here the first sequences for vent annelid hsp70 (P. grasslei, Hesiolyra bergi and Alvinella pompejana). These constitute valuable tools for future studies on the thermal biology of these annelids.

Key words: Hydrothermal vents, heat shock proteins, stress response, heat stress, annelids, IPOCAMP


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