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First published online December 14, 2007
Journal of Experimental Biology 211, 92-105 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.012450

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Conservation of structure, signaling and pharmacology between two serotonin receptor subtypes from decapod crustaceans, Panulirus interruptus and Procambarus clarkii

Nadja Spitzer^*, Donald H. Edwards and Deborah J. Baro

Department of Biology, Georgia State University, Atlanta, GA 30302, USA

Author for correspondence (e-mail: dbaro{at}gsu.edu)

Accepted 25 October 2007

Serotonin (5-HT) plays important roles in the maintenance and modulation of neural systems throughout the animal kingdom. The actions of 5-HT have been well characterized for several crustacean model circuits; however, a dissection of the serotonergic transduction cascades operating in these models has been hampered by the lack of pharmacological tools for invertebrate receptors. Here we provide pharmacological profiles for two 5-HT receptors from the swamp crayfish, Procambarus clarkii: 5-HT_2β and 5-HT₁. In so doing, we also report the first functional expression of a crustacean 5-HT₁ receptor, and show that it inhibits accumulation of cAMP. The drugs mCPP and quipazine are 5-HT₁ agonists and are ineffective at 5-HT_2β. Conversely, methiothepin and cinanserin are antagonists of 5-HT_2β but do not block 5-HT₁. A comparison of these two receptors with their orthologs from the California spiny lobster, Panulirus interruptus, indicates conservation of protein structure, signaling and pharmacology. This conservation extends beyond crustacean infraorders. The signature residues that form the ligand-binding pocket in mammalian 5-HT receptors are found in the crustacean receptors. Similarly, the protein domains involved in G protein coupling are conserved between the two crustacean receptors and other characterized arthropod and mammalian 5-HT receptors. Considering the apparent conservation of pharmacological properties between crustacean 5-HT receptors, these tools could be applicable to related crustacean physiological preparations.

Key words: agonist, antagonist, neuromodulation, G protein-coupled receptor, amine, cloning

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