|
| ![]() |
|
||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online June 15, 2007
Journal of Experimental Biology 210, 2346-2351 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.002857
Physiological regulation of glucose transporter (GLUT4) protein content in brown trout (Salmo trutta) skeletal muscle

Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain
Author for correspondence (e-mail:
jplanas{at}ub.edu)
Accepted 18 April 2007
In brown trout, red and white skeletal muscle express the insulin-regulatable glucose transporter 4 (btGLUT4). We have previously shown that the mRNA expression of btGLUT4 in red muscle, but not white muscle, is altered under experimental conditions designed to cause changes in the plasma levels of insulin, such as fasting, insulin and arginine administration. In order to determine whether changes of btGLUT4 expression at the mRNA level are correlated with changes at the protein level, we performed in vivo experiments to alter blood insulin concentrations and determined the abundance of btGLUT4 protein in trout red and white skeletal muscle by immunoblotting using an antibody to salmon GLUT4. In the present study we show that btGLUT4 protein content in red muscle decreases after fasting and increases after insulin administration. By contrast, btGLUT4 protein content in white muscle decreases after fasting but is not affected by insulin treatment. Our results show a good correlation between the changes observed in btGLUT4 protein and the previously reported changes in mRNA levels in response to alterations in circulating insulin, indicating that the regulation of btGLUT4 in brown trout takes place predominantly in the red skeletal muscle.
Key words: GLUT4, insulin, muscle, protein, trout
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati
Twitter What's this?
This article has been cited by other articles:
![]() |
S. Panserat, S. Skiba-Cassy, I. Seiliez, M. Lansard, E. Plagnes-Juan, C. Vachot, P. Aguirre, L. Larroquet, G. Chavernac, F. Medale, et al. Metformin improves postprandial glucose homeostasis in rainbow trout fed dietary carbohydrates: a link with the induction of hepatic lipogenic capacities? Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2009; 297(3): R707 - R715. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Diaz, Y. Vraskou, J. Gutierrez, and J. V. Planas Expression of rainbow trout glucose transporters GLUT1 and GLUT4 during in vitro muscle cell differentiation and regulation by insulin and IGF-I Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2009; 296(3): R794 - R800. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. Plagnes-Juan, M. Lansard, I. Seiliez, F. Medale, G. Corraze, S. Kaushik, S. Panserat, and S. Skiba-Cassy Insulin regulates the expression of several metabolism-related genes in the liver and primary hepatocytes of rainbow trout (Oncorhynchus mykiss) J. Exp. Biol., August 1, 2008; 211(15): 2510 - 2518. [Abstract] [Full Text] [PDF] |
||||