spacer gif spacer gif spacer gif spacer gif Propose a Workshop for 2011 spacer gif
 QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online June 15, 2007
Journal of Experimental Biology 210, 2253-2266 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.005116
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JEB
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Podrabsky, J. E.
Right arrow Articles by Somero, G. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Podrabsky, J. E.
Right arrow Articles by Somero, G. N.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*LACTIC ACID
*SUCCINIC ACID
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus: insights from a metabolomics analysis

Jason E. Podrabsky1,*, James P. Lopez2, Teresa W. M. Fan3, Richard Higashi3 and George N. Somero4

1 Department of Biology, Portland State University, PO Box 751, Portland, OR 97207-0751, USA
2 Department of Neurobiology, Pharmacology, and Physiology, The University of Chicago, Chicago, IL 60637, USA
3 Department of Chemistry, Belknap Research Building, 2210 S. Brook Street, University of Louisville, Louisville, KY 40208, USA
4 Hopkins Marine Station, Stanford University, Oceanview Boulevard, Pacific Grove, CA 93950, USA

* Author for correspondence (e-mail: jpod{at}pdx.edu)

Accepted 5 April 2007

The annual killifish Austrofundulus limnaeus survives in ephemeral pond habitats by producing drought-tolerant diapausing embryos. These embryos probably experience oxygen deprivation as part of their normal developmental environment. We assessed the anoxia tolerance of A. limnaeus embryos across the duration of embryonic development. Embryos develop a substantial tolerance to anoxia during early development, which peaks during diapause II. This extreme tolerance of anoxia is retained during the first 4 days of post-diapause II development and is then lost. Metabolism during anoxia appears to be supported mainly by production of lactate, with alanine and succinate production contributing to a lesser degree. Anoxic embryos also accumulate large quantities of {gamma}-aminobutyrate (GABA), a potential protector of neural function. It appears that the suite of characters associated with normal development and entry into diapause II in this species prepares the embryos for long-term survival in anoxia even while the embryos are exposed to aerobic conditions. This is the first report of such extreme anoxia tolerance in a vertebrate embryo, and introduces a new model for the study of anoxia tolerance in vertebrates.

Key words: anaerobiosis, anoxia, development, fish, GABA, lactate, metabolic depression


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JEB:

KILLIFISH: CHAMPION ANOXIA SURVIVORS
Laura Blackburn
JEB 2007 210: ii. [Full Text]  



This article has been cited by other articles:


Home page
J. Exp. Biol.Home page
J. A. Reynolds and S. C. Hand
Embryonic diapause highlighted by differential expression of mRNAs for ecdysteroidogenesis, transcription and lipid sparing in the cricket Allonemobius socius
J. Exp. Biol., July 1, 2009; 212(13): 2075 - 2084.
[Abstract] [Full Text] [PDF]


Home page
NeuroscientistHome page
R. Meller
The Role of the Ubiquitin Proteasome System in Ischemia and Ischemic Tolerance
Neuroscientist, June 1, 2009; 15(3): 243 - 260.
[Abstract] [PDF]


Home page
Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Tingaud-Sequeira, C. Zapater, F. Chauvigne, D. Otero, and J. Cerda
Adaptive plasticity of killifish (Fundulus heteroclitus) embryos: dehydration-stimulated development and differential aquaporin-3 expression
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1041 - R1052.
[Abstract] [Full Text] [PDF]


Home page
Physiol. GenomicsHome page
A. R. Mendenhall, M. G. LeBlanc, D. P. Mohan, and P. A. Padilla
Reduction in ovulation or male sex phenotype increases long-term anoxia survival in a daf-16-independent manner in Caenorhabditis elegans
Physiol Genomics, February 2, 2009; 36(3): 167 - 178.
[Abstract] [Full Text] [PDF]


Home page
J. Exp. Biol.Home page
L. Blackburn
KILLIFISH: CHAMPION ANOXIA SURVIVORS
J. Exp. Biol., July 1, 2007; 210(13): ii - ii.
[Full Text] [PDF]




© The Company of Biologists Ltd 2007