QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by DE MEILLON, B. Articles by LAVOIPIERRE, M. Search for Related Content PubMed Articles by DE MEILLON, B. Articles by LAVOIPIERRE, M. Social Bookmarking                         What's this?

Journal of Experimental Biology 21,84-89 (1945)
Published by Company of Biologists 1945

The Nutrition of the Larva of Aedes Aegypti L. I

B. DE MEILLON D.Sc., F.R.E.S.¹, L. GOLBERG M.SC., D.PHIL., A.R.I.C.¹, and M. LAVOIPIERRE B.SC.¹

¹ South African Institute for Medical Research, Johannesburg, South Africa

1. A technique for investigating the nutritional requirements of the larva of Aedes aegypti, under sterile conditions, is described. A method for more accurate recording and interpretation of results is presented.

2. First-stage larvae may be kept in agglutinating broth for periods up to 96 hr. without affecting their subsequent growth in suitable media.

3. A suspension of autoclaved brewer's yeast in calcium chloride solution supports growth and metamorphosis of the larvae. The addition of filtered liver extract greatly improves the growth and survival rates. Further addition of a mixture of vitamins of the B group brings about no corresponding further improvement; neither does replacement of the liver extract by papaic liver digest with or without the vitamin mixture.

4. Storage of dried brewer's yeast for a long period, or of autoclaved fresh brewer's yeast for a shorter period, has a marked adverse effect on the growth rate when these yeasts are combined with liver extract or vitamin mixture. A similar effect is brought about by prologned autoclaving of the yeasts.

5. In all these media liver extract may be repalced by the mixture of B vitamins. It was not found possible, however, to replace the solid yeast fraction of the medium by any combination of substances so far investigated.

6. The time taken for the change from pupa to adult is independent of the medium in which the larva was grown.

7. There is a factor which causes the emergence of vigorous adults in nature or in contaminated media. This factor has been found to be largely absent from our best media such as brewer's yeast and liver.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?