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First published online January 19, 2006
Journal of Experimental Biology 209, 518-530 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02018
The role of branchial carbonic anhydrase in acid-base regulation in rainbow trout (Oncorhynchus mykiss)
Department of Biology and Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada
* Author for correspondence (e-mail: Katie.Gilmour{at}science.uottawa.ca)
Accepted 29 November 2005
The objective of the present study was to examine the branchial
distribution of the recently identified rainbow trout cytoplasmic carbonic
anhydrase isoform (tCAc) and to investigate its role in the regulation of
acid-base disturbances in rainbow trout (Oncorhynchus mykiss). In
situ hybridization using an oligonucleotide probe specific to tCAc
revealed tCAc mRNA expression in both pavement cells and mitochondria-rich
cells (chloride cells). Similarly, using a homologous polyclonal antibody,
tCAc immunoreactivity was localized to pavement cells and mitochondria-rich
cells in the interlamellar region and along the lamellae of the gills.
Exposure of rainbow trout to hypercarbia (
0.8% CO2) for 24 h
resulted in significant increases in tCAc mRNA expression (20-fold;
quantified by real-time PCR) and protein levels (1.3-fold; quantified by
western analysis) but not enzyme activity (assessed on crude gill homogenates
using the delta-pH CA assay). Inhibition of branchial CA activity in
vivo using acetazolamide reduced branchial net acid excretion
significantly by 20%. This effect was enhanced to a 36% reduction in branchial
net acid excretion by subjecting the trout to hypercarbia (0.8%
CO2) for 10 h prior to acetazolamide injection, an exposure that
significantly increased branchial net acid excretion. The results of the
present study support the widely held premise that branchial intracellular CA
activity (tCAc) plays a key role in regulating acid-base balance in freshwater
teleost fish.
Key words: carbonic anhydrase, acid-base balance, gill, rainbow trout, acetazolamide, hypercarbia, acid excretion
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