|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online November 17, 2006
Journal of Experimental Biology 209, 4638-4651 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02551
P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquito Aedes aegypti
Department of Cell Biology and Neuroscience, University of California, Riverside, CA 92521-0146, USA
* Author for correspondence at present address: Department of Biology, University of San Diego, 5998 Alcalá Park, San Diego, CA 92111, USA (e-mail: mpatrick{at}sandiego.edu)
Accepted 13 September 2006
This study describes the expression patterns of P-type Na+/K+-ATPase and V-type H+-ATPase in the larval and adult forms of the mosquito Aedes aegypti and provides insight into their relative importance in ion transport function of key osmoregulatory organs. RT-PCR assays indicate that, at the level of the gene, both ATPases are expressed in all of the osmoregulatory tissues of larvae (midgut, Malpighian tubules, rectum and anal papillae) and adults (stomach, Malpighian tubules, anterior hindgut and rectum). Immunohistochemical studies determined that both ATPases are present in high levels in all the relevant organs, with the exception of the larval rectum (P-type Na+/K+-ATPase only). In larval gastric caeca, ATPase location corresponds to the secretory (basal P-type Na+/K+-ATPase, apical V-type H+-ATPase) and ion-transporting (V-type H+-ATPase on both membranes) regions as previously described. The two ATPases switch membrane location along the length of the larval midgut, indicating three possible regionalizations, whereas the adult stomach has uniform expression of basolateral P-type Na+/K+-ATPase and apical V-type H+-ATPase in each cell. In both larval and adult Malpighian tubules, the distal principal cells exhibit high expression levels of V-type H+-ATPase (apically and cytoplasmically) whereas P-type Na+/K+-ATPase is highly expressed in stellate cells found only in the distal two-thirds of each tubule. By contrast, the proximal principal cells express both P-type Na+/K+-ATPase (basal) and V-type H+-ATPase (apical). These results suggest a functional segregation along the length of the Malpighian tubules based on cell type and region. P-type Na+/K+-ATPase is the only pump apparent in the larval rectum whereas in the larval anal papillae and the adult hindgut (including the anterior hindgut and rectum with rectal pads), P-type Na+/K+-ATPase and V-type H+-ATPase localize to the basal and apical membranes, respectively. We discuss our findings in light of previous physiological and morphological studies and re-examine our current models of ion transport in these two developmental stages of mosquitoes that cope with disparate osmoregulatory challenges.
Key words: mosquito, larva, adult, Aedes aegypti, P-type Na+/K+-ATPase, V-type H+-ATPase, ion transport, osmoregulation
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
Related articles in JEB:
This article has been cited by other articles:
|
|
 |
|
  A. M. Evans, K. G. Aimanova, and S. S. Gill Characterization of a blood-meal-responsive proton-dependent amino acid transporter in the disease vector, Aedes aegypti J. Exp. Biol., October 15, 2009; 212(20): 3263 - 3271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shanbhag and S. Tripathi Epithelial ultrastructure and cellular mechanisms of acid and base transport in the Drosophila midgut J. Exp. Biol., June 1, 2009; 212(11): 1731 - 1744. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Piermarini, D. Weihrauch, H. Meyer, M. Huss, and K. W. Beyenbach NHE8 is an intracellular cation/H+ exchanger in renal tubules of the yellow fever mosquito Aedes aegypti Am J Physiol Renal Physiol, April 1, 2009; 296(4): F730 - F750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Stergiopoulos, P. Cabrero, S.-A. Davies, and J. A. T. Dow Salty dog, an SLC5 symporter, modulates Drosophila response to salt stress Physiol Genomics, March 3, 2009; 37(1): 1 - 11. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Harvey, D. Y. Boudko, M. R. Rheault, and B. A. Okech NHEVNAT: an H+ V-ATPase electrically coupled to a Na+:nutrient amino acid transporter (NAT) forms an Na+/H+ exchanger (NHE) J. Exp. Biol., February 1, 2009; 212(3): 347 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Onken and D. F. Moffett Revisiting the cellular mechanisms of strong luminal alkalinization in the anterior midgut of larval mosquitoes J. Exp. Biol., February 1, 2009; 212(3): 373 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Smith, L. A. VanEkeris, B. A. Okech, W. R. Harvey, and P. J. Linser Larval anopheline mosquito recta exhibit a dramatic change in localization patterns of ion transport proteins in response to shifting salinity: a comparison between anopheline and culicine larvae J. Exp. Biol., October 1, 2008; 211(19): 3067 - 3076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Okech, D. Y. Boudko, P. J. Linser, and W. R. Harvey Cationic pathway of pH regulation in larvae of Anopheles gambiae J. Exp. Biol., March 15, 2008; 211(6): 957 - 968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Smith, L. A. VanEkeris, and P. J. Linser Cloning and characterization of AgCA9, a novel {alpha}-carbonic anhydrase from Anopheles gambiae Giles sensu stricto (Diptera: Culicidae) larvae J. Exp. Biol., November 15, 2007; 210(22): 3919 - 3930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Rheault, B. A. Okech, S. B. W. Keen, M. M. Miller, E. A. Meleshkevitch, P. J. Linser, D. Y. Boudko, and W. R. Harvey Molecular cloning, phylogeny and localization of AgNHA1: the first Na+/H+ antiporter (NHA) from a metazoan, Anopheles gambiae J. Exp. Biol., November 1, 2007; 210(21): 3848 - 3861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Donini, M. P. Gaidhu, D. R. Strasberg, and M. J. O'Donnell Changing salinity induces alterations in hemolymph ion concentrations and Na+ and Cl- transport kinetics of the anal papillae in the larval mosquito, Aedes aegypti J. Exp. Biol., March 15, 2007; 210(6): 983 - 992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Blackburn MOSQUITOES KEEP THE BALANCE J. Exp. Biol., December 1, 2006; 209(23): ii - ii. [Full Text] [PDF]
|
|
