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First published online October 5, 2006
Journal of Experimental Biology 209, 3964-3973 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02463

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Selection on knockdown performance in Drosophila melanogaster impacts thermotolerance and heat-shock response differently in females and males

Donna G. Folk^1,*, Patty Zwollo¹, David M. Rand² and George W. Gilchrist¹

¹ Department of Biology, College of William and Mary, Williamsburg, VA 23187, USA
² Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, USA

^* Author for correspondence (e-mail: dgfolk{at}wm.edu)

Accepted 31 July 2006

We studied adaptive thermotolerance in replicate populations of Drosophila melanogaster artificially selected for high and low knockdown temperature (T_KD), the upper temperature at which flies can no longer remain upright or locomote effectively. Responses to selection have generated High T_KD populations capable of maintaining locomotor function at 40°C, and Low T_KD populations with T_KD of 35°C. We examined inducible knockdown thermotolerance, as well as inducible thermal survivorship, following a pretreatment heat-shock (known to induce heat-shock proteins) for males and females from the T_KD selected lines. Both selection for knockdown and sex influenced inducible knockdown thermotolerance, whereas inducible thermal survivorship was influenced only by sex, and not by selection. Overall, our findings suggest that the relationships between basal and inducible thermotolerance are contingent upon the methods used to gauge thermotolerance, as well as the sex of the flies. Finally, we compared temporal profiles of the combined expression of two major heat-shock proteins, HSC70 and HSP70, during heat stress among the females and males from the selected T_KD lines. The temporal profiles of the proteins differed between High and Low T_KD females, suggesting divergence of the heat-shock response. We discuss a possible mechanism that may lead to the heat-shock protein patterns observed in the selected females.

Key words: Drosophila melanogaster, laboratory selection, knockdown, thermotolerance, heat-shock response, HSC70, HSP70

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