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First published online September 19, 2006
Journal of Experimental Biology 209, 3719-3728 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02436

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Dietary acidification enhances phosphorus digestibility but decreases H⁺/K⁺-ATPase expression in rainbow trout

Shozo H. Sugiura, Prabir K. Roy and Ronaldo P. Ferraris^*

Department of Pharmacology and Physiology, UMDNJ-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA

^* Author for correspondence (e-mail: ferraris{at}umdnj.edu)

Accepted 11 July 2006

Oxynticopeptic cells of fish stomach are thought to secrete less acid than the specialized parietal cells of mammalian stomach. Gastric acidity, however, has not been directly compared between fish and mammals. We therefore fed rainbow trout and rats the same meal, and found that the lowest postprandial pH of trout stomach was 2.7, which was only transiently sustained for 1 h, whereas that of rat stomach was 1.3, which was sustained for 3 h. Postprandial pH of the small intestine was slightly higher in trout (8.0) than in rats (7.6), but pH of the large intestine was similar (8.0). Addition of acids to fish feeds, in an attempt to aid the weak acidity of fish stomach, has been known to improve phosphorus digestibility, but its physiological effect on fish stomach is not known. Exogenous acids did improve phosphorus digestibility but also decreased steady-state mRNA expression of trout H⁺/K⁺-ATPase (ATP4A, the proton pump) as well as Na⁺/bicarbonate cotransporter (NBC), and had no effect on gastrin-like mRNA and somastostatin (SST) mRNA abundance. Gastrin-like mRNA and SST-2 mRNA were equally distributed between corpus and antrum. ATP4A mRNA and NBC mRNA were in the corpus, whereas SST-1 mRNA was in the antrum. Trout gastrin-like EST had modest homology to halibut and pufferfish gastrin, whereas trout ATP4A mRNA had 95% amino acid homology with mammalian, Xenopus and flounder ATP4A. Although ATP4A seems highly conserved among vertebrates, gastric acidity is much less in trout than in rats, explaining the low digestibility of bone phosphorus, abundant in fish diets. Dietary acidification does not reduce acidity enough to markedly improve phosphorus digestibility, perhaps because exogenous acids may inhibit endogenous acid production.

Key words: gastrin, oxynticopeptic cells, proton pump, somatostatin, stomach pH